Lobe C G, Upton C, Duggan B, Ehrman N, Letellier M, Bell J, McFadden G, Bleackley R C
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Biochemistry. 1988 Sep 6;27(18):6941-6. doi: 10.1021/bi00418a040.
The genes encoding two recently described cytotoxic T cell proteases, CCPI and CCPII, have been isolated and sequenced. The organizations of the coding and noncoding portions of the two genes are very similar to each other and also to the gene encoding rat mast cell protease type II. Similarly to other serine protease genes, each of the active-site residues is contained on a separate exon; however, two introns were found in particularly interesting positions. One occurs within the postulated activation dipeptide and the other in a position close to the active-site Asp residue. This latter intron interrupts the amino acid sequence in the invariant core region of the protein. We believe that these genes represent a new subfamily of serine protease genes.
编码最近发现的两种细胞毒性T细胞蛋白酶CCPI和CCPII的基因已被分离并测序。这两个基因的编码和非编码部分的结构彼此非常相似，并且与编码大鼠II型肥大细胞蛋白酶的基因也很相似。与其他丝氨酸蛋白酶基因一样，每个活性位点残基都位于一个单独的外显子上；然而，在特别有趣的位置发现了两个内含子。一个位于假定的激活二肽内，另一个位于靠近活性位点天冬氨酸残基的位置。后一个内含子打断了蛋白质不变核心区域的氨基酸序列。我们认为这些基因代表了丝氨酸蛋白酶基因的一个新亚家族。
