Prendergast J A, Pinkoski M, Wolfenden A, Bleackley R C
Department of Biochemistry, University of Alberta, Edmonton, Canada.
J Mol Biol. 1991 Aug 20;220(4):867-75. doi: 10.1016/0022-2836(91)90359-e.
A family of serine proteinases is believed to be important in cell-mediated cytotoxicity. Presented here are the genomic sequences for three murine members of this cytotoxic cell proteinase (CCP) family: the CCP3, CCP4 and CCP5 genes. All three of these genes have introns inserted at the same codon sites and the same exon distribution of the active site residues. These characteristics are also shared with the CCP1 and CCP2 genes, the charter members of the CCP gene family. Phylogenetic analysis using intron and exon sequences suggests that all five genes arose by various duplication events. This analysis also indicates that the recently described HuCCPX and CCP2 genes originated from recombination events between genes of different lineages. A phylogenetic and Southern analysis of the recombinant HuCCPX gene suggests that the human genome contains an additional CCP gene that has yet to be described. Finally, evidence is presented suggesting that the cDNA clone originally describing the CCP5 gene was derived from an alternately spliced transcript.
人们认为，丝氨酸蛋白酶家族在细胞介导的细胞毒性中起重要作用。本文展示了该细胞毒性细胞蛋白酶（CCP）家族的三个小鼠成员的基因组序列：CCP3、CCP4和CCP5基因。这三个基因的内含子均插入在相同的密码子位点，且活性位点残基的外显子分布相同。这些特征也与CCP基因家族的首批成员CCP1和CCP2基因相同。使用内含子和外显子序列进行的系统发育分析表明，所有五个基因均通过各种复制事件产生。该分析还表明，最近描述的HuCCPX和CCP2基因起源于不同谱系基因之间的重组事件。对重组HuCCPX基因的系统发育和Southern分析表明，人类基因组中含有另一个尚未描述的CCP基因。最后，有证据表明最初描述CCP5基因的cDNA克隆源自一个可变剪接的转录本。
