Hedayatyanfard Keshvad, Bagheri-Khoulenjani Shadab, Hashemi Ali, Ziai Seyed Ali
Students Research Committee, Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Polymer Engineering, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Iran J Pharm Res. 2019 Summer;18(3):1156-1167. doi: 10.22037/ijpr.2019.1100712.
The antimicrobial activity of a wound dressing is a key factor for preventing and treating wound infection. The current study evaluated the physiochemical properties and antimicrobial activities of semi-IPNs (interpenetrating polymer networks) based on chitosan/polyvinyl alcohol (PVA) films and nanofibers as candidates for wound dressings and investigated the effects of morphologies (nanofibrous mats and films), crosslinking conditions of chitosan chains (uncrosslinked and crosslinked with genipin), and the presence of antibacterial drug (doxycycline) on their physicochemical and antibacterial properties. The morphology, chemical structure, fluid uptake, water vapor transmission rate, antimicrobial activity, and doxycycline release profile were assayed using SEM, FTIR spectroscopy, swelling test, permeation test, agar diffusion antibiogram, and dissolution test, respectively. The results demonstrated that crosslinking chitosan with genipin reduced the diameter of nanofibers, fluid uptake, and drug release from both nanofiber mats and film samples. According to the results, wound dressings with film morphology have better antimicrobial activity than those with nanofiber. The chitosan/PVA/Doxycycline 1% film has the potential for use as an antimicrobial wound dressing.
伤口敷料的抗菌活性是预防和治疗伤口感染的关键因素。本研究评估了基于壳聚糖/聚乙烯醇（PVA）薄膜和纳米纤维的半互穿聚合物网络（semi-IPNs）作为伤口敷料候选材料的物理化学性质和抗菌活性，并研究了形态（纳米纤维垫和薄膜）、壳聚糖链的交联条件（未交联和与京尼平交联）以及抗菌药物（强力霉素）的存在对其物理化学和抗菌性能的影响。分别使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、溶胀试验、渗透试验、琼脂扩散抑菌圈试验和溶出试验对形态、化学结构、吸液性、水蒸气透过率、抗菌活性和强力霉素释放曲线进行了测定。结果表明，壳聚糖与京尼平交联可减小纳米纤维的直径、降低纳米纤维垫和薄膜样品的吸液性和药物释放。根据结果，薄膜形态的伤口敷料比纳米纤维形态的具有更好的抗菌活性。1%的壳聚糖/PVA/强力霉素薄膜有潜力用作抗菌伤口敷料。
