猪胰腺中α-淀粉酶的同工酶：群体分布

Isozymes of alpha-amylase in the porcine pancreas: population distribution.

Strumeyer D H, Kao W, Roberts T, Forsyth-Davis D

Department of Biochemistry and Microbiology, Rutgers, State University of New Jersey, New Brunswick 08903.

Comp Biochem Physiol B. 1988;91(2):351-7. doi: 10.1016/0305-0491(88)90153-8.

Abstract

Three isozymes of pancreatic alpha-amylase, PPA 1, PPA 2, and PPA 3, were observed in a porcine population of 50 animals. 2. Isozyme PPA 2 was common to each pancreas. 3. Three phenotypic patterns were described as: (A) consisting of PPA 2 alone (20%); (B) consisting of PPA 1 and PPA 2 (78%); and (C) consisting of all three forms (2%). 4. Amylase isozymes were separated by anion exchange chromatography using DE53. 5. Individual isozymes corresponded to one of the three isozymes found in pancreatin. 6. Individual isozymes were inhibited equally by an amylase inhibitor from wheat. 7. Differences in amylase isozymes were attributed to genetically controlled mechanisms and not to artifacts of isolation.

摘要

在一个由50头猪组成的群体中观察到了胰腺α-淀粉酶的三种同工酶，即PPA 1、PPA 2和PPA 3。2. 同工酶PPA 2在每一个胰腺中都存在。3. 描述了三种表型模式：(A) 仅由PPA 2组成（20%）；(B) 由PPA 1和PPA 2组成（78%）；以及(C) 由所有三种形式组成（2%）。4. 使用DE53通过阴离子交换色谱法分离淀粉酶同工酶。5. 个体同工酶与胰酶中发现的三种同工酶之一相对应。6. 个体同工酶受到来自小麦的淀粉酶抑制剂的同等抑制。7. 淀粉酶同工酶的差异归因于遗传控制机制，而非分离假象。