来自人胰液的α淀粉酶作为一种电泳纯同工酶。

alpha-Amylase from human pancreatic juice as an electrophoretically pure isozyme.

作者信息

Matsuura K, Ogawa M, Kosaki G, Minamiura N, Tamamoto T

出版信息

J Biochem. 1978 Jan;83(1):329-32. doi: 10.1093/oxfordjournals.jbchem.a131909.

DOI:10.1093/oxfordjournals.jbchem.a131909

Abstract

Human pancreatic juice collected antiseptically and strictly free from other body fluids and juices, was examined for the isozyme composition of alpha-amylase. Unlike those of the extract of and exudate from pancreatic tissue, the composition of alpha-amylase isozyme in the secreted juice was simple and consisted of only two isozymes, a major one and a minor one, and the reason for difference in the isozyme composition is discussed. The major alpha-amylase isozyme was obtained in a crystalline state with a molecular weight of 5.4-5.5 X 10(4).

摘要

无菌采集且严格不含其他体液和汁液的人胰液，被检测了α-淀粉酶的同工酶组成。与胰腺组织提取物和渗出液中的情况不同，分泌液中α-淀粉酶同工酶的组成很简单，仅由两种同工酶组成，一种主要的和一种次要的，并对同工酶组成差异的原因进行了讨论。主要的α-淀粉酶同工酶以结晶状态获得，分子量为5.4 - 5.5×10⁴。