Watts D I, Monteiro M J, Cox R A
Laboratory of Developmental Biochemistry, National Institute for Medical Research, London, England.
FEBS Lett. 1988 Dec 5;241(1-2):229-33. doi: 10.1016/0014-5793(88)81067-6.
The N alpha-tubulin gene of Physarum polycephalum has an EcoRV site at codons 252/253. EcoRV digestion of physarum DNA generated two EcoRV fragments per gene copy comprising both coding and flanking sequences. Hybridisation probes which included coding sequences upstream from the central EcoRV site cross-hybridised with another alpha-tubulin gene. Probes derived from either 5'- or 3'-flanking regions were gene-specific. These probes identified two EcoRV fragments in the haploid strain CLdAXE viz 5.4 kb (5'-fragment) and 6.2 kb (3'-fragment). The same two fragments were identified in EcoRV digests of DNA of the diploid strain M3CVIII, and a second form of the gene was also identified comprising two fragments viz 5.0 kb (5'-end) and 5.5 kb (3'-end). Both forms gave rise to an identical 4.65 kb HindIII fragment as judged by restriction mapping.
多头绒泡菌的Nα-微管蛋白基因在密码子252/253处有一个EcoRV位点。用EcoRV消化多头绒泡菌DNA，每个基因拷贝产生两个EcoRV片段，包含编码序列和侧翼序列。包含中央EcoRV位点上游编码序列的杂交探针与另一个α-微管蛋白基因发生交叉杂交。来自5'或3'侧翼区域的探针具有基因特异性。这些探针在单倍体菌株CLdAXE中鉴定出两个EcoRV片段，即5.4 kb（5'-片段）和6.2 kb（3'-片段）。在二倍体菌株M3CVIII的DNA的EcoRV消化物中也鉴定出了相同的两个片段，并且还鉴定出了该基因的第二种形式，由两个片段组成，即5.0 kb（5'-末端）和5.5 kb（3'-末端）。通过限制性图谱分析判断，这两种形式都产生了相同的4.65 kb HindIII片段。
