Jones E P, Mahendran R, Spottswood M R, Yang Y C, Miller D L
Cell and Molecular Biology Program, University of Texas, Dallas, Richardson 75083-0688.
Curr Genet. 1990 Apr;17(4):331-7. doi: 10.1007/BF00314881.
Mitochondrial DNA (mtDNA) has been isolated from four strains of Physarum polycephalum and a restriction site map has been determined using nine restriction enzymes. The restriction site maps of the four strains are similar but each strain is distinguished by insertions, deletions and restriction enzyme site polymorphisms. The sum of the restriction fragments gives mitochondrial genome sizes which vary from about 56 kb to 62 kb. In all four strains the composite map of the restriction enzyme sites for the mtDNA is circular. Knowledge of the restriction enzyme map has enabled cloning of mtDNA fragments representing the entire mtDNA of strain M3. The cloned fragments have been used to create a transcription map of the mtDNA.
已从多头绒泡菌的四个菌株中分离出线粒体DNA（mtDNA），并使用九种限制酶确定了限制酶切位点图谱。这四个菌株的限制酶切位点图谱相似，但每个菌株都有插入、缺失和限制酶切位点多态性的特征。限制片段的总和给出了线粒体基因组大小，其范围从约56 kb到62 kb不等。在所有四个菌株中，mtDNA的限制酶切位点复合图谱都是环状的。限制酶图谱的知识使得能够克隆代表菌株M3整个mtDNA的mtDNA片段。这些克隆片段已被用于构建mtDNA的转录图谱。
