Canive Maria, Casais Rosa, Jimenez Jose A, Blanco-Vazquez Cristina, Amado Javier, Garrido Joseba M, Juste Ramon A, Alonso-Hearn Marta
NEIKER- Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Department of Animal Health, Derio, Bizkaia, Spain.
Center of Animal Biotechnology, SERIDA-Regional Service of Agri-Food Research and Development, Deva, Asturias, Spain.
Heliyon. 2020 Jun 30;6(6):e04254. doi: 10.1016/j.heliyon.2020.e04254. eCollection 2020 Jun.
Little is known about the correlations between the genetic susceptibility/resistance to ubsp. ( infection and the estimated breeding values for type, production and functional traits. Previously, we identified 70 combinations of five single nucleotide polymorphisms (SNPs) in four bovine innate immune genes (, , , ) that are associated with the genetic risk of paratuberculosis (PTB) or Johne's disease progression, which can be graded as low (LOWIN), latent (LATIN), or patent (PATIN) risk. Other possible combinations of these 5 SNPs were grouped in the average group (AVERIN). In the current study, differences in estimated breeding values (EBVs) for several traits were analyzed using linear models in a large cohort of Holstein cows (N = 15656) genotyped across Spain in 2016 or 2017. After the assignment of each genotyped cow to a risk group, cows within the PATIN risk group (N = 1448) had a superior combined genetic index (2797.57), type genetic index (524.62), milk yield (653.92 kg), protein yield (21.77 kg), fat yield (24.82 kg) and economic merit index (125 Euros) compared with the other three risk groups. Statistically significant differences in the longevity scores between the cows that were included in the PATIN risk group (108.85) and the LOWIN (107.82) and AVERIN (107.92) groups were also observed. The associations between the genetic risk groups and PTB diagnostic results were validated in a population of 99 cows from a Spanish farm with a high prevalence of PTB. Significant differences in ELISA readings between the PATIN (65.49 %) and the AVERIN (15.97 %), LATIN (2.11 %), and LOWIN (3.27 %) groups were observed. In addition, significant differences in DNA copies/gram of feces were observed between the PATIN and the other three risk groups. These results together with the substantial economic impact of PTB in dairy cattle support the selection of the animals with less susceptibility to PTB in the Spanish breeding program.
关于对副结核分枝杆菌感染的遗传易感性/抗性与类型、生产和功能性状的估计育种值之间的相关性，人们了解甚少。此前，我们在四个牛先天免疫基因中鉴定出五个单核苷酸多态性（SNP）的70种组合，这些组合与副结核病（PTB）或约翰氏病进展的遗传风险相关，其可分为低风险（LOWIN）、潜伏风险（LATIN）或显性风险（PATIN）。这5个SNP的其他可能组合被归为平均组（AVERIN）。在本研究中，使用线性模型分析了2016年或2017年在西班牙进行基因分型的一大群荷斯坦奶牛（N = 15656）中几个性状的估计育种值（EBV）差异。在将每头基因分型奶牛分配到一个风险组后，PATIN风险组（N = 1448）内的奶牛在综合遗传指数（2797.57）、类型遗传指数（524.62）、产奶量（653.92千克）、蛋白质产量（21.77千克）、脂肪产量（24.82千克）和经济价值指数（125欧元）方面优于其他三个风险组。在PATIN风险组（108.85）与LOWIN（107.82）和AVERIN（（107.92）组的奶牛之间的长寿得分上也观察到统计学上的显著差异。在西班牙一个PTB高流行农场的99头奶牛群体中验证了遗传风险组与PTB诊断结果之间的关联。在PATIN（65.49%）与AVERIN（15.97%）、LATIN（2.11%）和LOWIN（3.27%）组之间观察到ELISA读数的显著差异。此外，在PATIN与其他三个风险组之间观察到粪便中每克DNA拷贝数的显著差异。这些结果以及PTB对奶牛的重大经济影响支持在西班牙育种计划中选择对PTB易感性较低的动物。
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