Okuni Julius Boniface, Afayoa Mathias, Ojok Lonzy
Department of Pharmacy, Veterinary Clinical and Companion Animal Medicine, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, Kampala, Uganda.
Department of Pathology, Faculty of Medicine, Gulu University, Gulu, Uganda.
Front Vet Sci. 2021 Feb 12;8:614518. doi: 10.3389/fvets.2021.614518. eCollection 2021.
ssp. (MAP) is the cause of Johne's disease (JD) in a wide range of domestic and wild ruminants. Single-nucleotide polymorphisms (SNPs) in several genes including solute-like carrier 11A1 (SLC11A1), interferon gamma (IFNγ), Toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain 2 gene (NOD2), and bovine peptidoglycan recognition protein 1 (PGLYRP1) have been implicated in influencing the infection outcome of MAP in cattle. We have carried out a survey in a population of Ankole cattle from three districts in the central region of Uganda including Isingiro, Lyantonde, and Rakai to determine the role played by several SNPs on the above genes in the infection outcome of local cattle in Uganda. Nine hundred fifty-five heads of cattle obtained from 93 herds were tested using ELISA. Thirty-five ELISA-positive cattle and 35 negative herd mates from a total of 955 cattle tested for MAP were genotyped using iPLEX MassARRAY genotyping systems to detect the presence of a total of 13 SNPS in five different genes (SLC11A1, IFNγ, TLR4, NOD2, and PGLYRP1). The cow-level prevalence of MAP infection in Ankole Longhorn cattle in the three districts was 3.98% (35/955), while the herd-level prevalence was 27.9% and within-herd prevalence was 12 ± 1.5% (95% CI = 9.1-14.8%). The genotypes and allele frequencies of the MAP-positive cattle were compared with those of their ELISA-negative herd mates to determine the significance of the polymorphisms. The results showed that SNPs rs109915208, rs110514940, and rs110905610 on SLC11A1, c.480G>A and c.625C>A on PGLYRP1, and c.2021C>T on TLR4 were monomorphic in both seropositive and seronegative cattle and therefore had no influence on the infection outcome. The remaining SNPs studied in the five genes [: rs109614179; : rs29017188 (c.226G>C), c.2021C>T; NOD2: rs110536091, rs111009394; : c.102G>C, c.480G>A, c.625C>A; γ: rs110853455] were polymorphic, but their allele and genotype frequencies did not show any significant difference between the seropositive and seronegative cattle. No significant difference was observed for any haplotype at the gene level.
副结核分枝杆菌（MAP）是多种家养和野生反刍动物患约内氏病（JD）的病因。包括溶质样载体11A1（SLC11A1）、干扰素γ（IFNγ）、Toll样受体4（TLR4）、核苷酸结合寡聚化结构域2基因（NOD2）和牛肽聚糖识别蛋白1（PGLYRP1）在内的多个基因中的单核苷酸多态性（SNP）与影响牛感染MAP的结果有关。我们对乌干达中部地区三个区（包括伊辛吉罗、利安通德和拉凯）的安科勒牛群进行了一项调查，以确定上述基因中的几个SNP对乌干达当地牛感染结果所起的作用。使用酶联免疫吸附测定（ELISA）对从93个牛群中获取的955头牛进行了检测。使用iPLEX MassARRAY基因分型系统对总共955头检测MAP的牛中35头ELISA阳性牛及其35头阴性同群牛进行基因分型，以检测五个不同基因（SLC11A1、IFNγ、TLR4、NOD2和PGLYRP1）中总共13个SNP的存在情况。这三个区的安科勒长角牛中MAP感染的个体水平患病率为3.98%（35/955），而群体水平患病率为27.9%，群内患病率为12±1.5%（95%置信区间=9.1 - 14.8%）。将MAP阳性牛的基因型和等位基因频率与其ELISA阴性同群牛的进行比较，以确定多态性的显著性。结果显示，SLC11A1上的SNP rs109915208、rs110514940和rs110905610，PGLYRP1上的c.480G>A和c.625C>A，以及TLR4上的c.2021C>T在血清阳性和血清阴性牛中均为单态性，因此对感染结果没有影响。在这五个基因中研究的其余SNP [：rs109614179；：rs29017188（c.226G>C），c.2021C>T；NOD2：rs110536091，rs111009394；：c.102G>C，c.480G>A，c.625C>A；γ：rs110853455]是多态性的，但它们的等位基因和基因型频率在血清阳性和血清阴性牛之间没有显示出任何显著差异。在基因水平上，任何单倍型均未观察到显著差异。
