Lambert B, Holmberg K, He S H, Einhorn N
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
IARC Sci Publ. 1988(89):469-76.
T-Lymphocyte clones from healthy males and females and from melphalan-treated ovarian carcinoma patients were studied with regard to sporadic chromosomal aberrations and clonal karyotype: 85% of the clones showed a normal, diploid karyotype, and sporadic aberrations were found to occur at about the same low frequency as in short-term lymphocyte cultures. An abnormal karyotype was found in 11 of the 72 clones studied. Loss of an X chromosome, which was the most frequent abnormality in female clones, was verified by densitometry of Southern blots of clonal DNA hybridized with a probe for the X-linked hprt locus. Abnormal karyotype due to chromosomal rearrangement was found in nine clones, and, in five of these, chromosome 12 was involved in the aberration. About 33% of the clones from melphalan-treated patients had an abnormal karyotype, in comparison with about 10% of clones from healthy control subjects. This difference indicated that melphalan treatment may induce stable chromosomal rearrangements that are compatible with cellular proliferation and clonal expansion.
对来自健康男性和女性以及美法仑治疗的卵巢癌患者的T淋巴细胞克隆进行了研究，观察其散发性染色体畸变和克隆核型：85%的克隆显示正常的二倍体核型，散发性畸变的发生频率与短期淋巴细胞培养中的频率大致相同。在所研究的72个克隆中，有11个发现核型异常。通过用X连锁的次黄嘌呤-鸟嘌呤磷酸核糖转移酶（hprt）基因座探针杂交的克隆DNA的Southern印迹密度测定法，证实了X染色体丢失是女性克隆中最常见的异常。在9个克隆中发现了由于染色体重排导致的异常核型，其中5个克隆的12号染色体参与了畸变。与健康对照受试者的约10%的克隆相比，美法仑治疗患者的约33%的克隆具有异常核型。这种差异表明美法仑治疗可能诱导与细胞增殖和克隆扩增相容的稳定染色体重排。
