奥地利人群中IgA缺乏症的发生率。一项通过酶联免疫吸附测定法进行大规模筛查的方案及对3056名献血者的研究。

The frequency of IgA-deficiency in the Austrian population. A protocol for large-scale screening by ELISA and a study on 3056 blood donors.

作者信息

Winter P M, Manndorff P

机构信息

Institut für Blutgruppenserologie, Universität Wien.

出版信息

Infusionstherapie. 1988 Oct;15(5):221-4.

PMID:3264271

Abstract

A protocol for large scale screening of blood donors for IgA-deficiency with the help of an ELISA is described. The ELISA method proved to be fast and reliable, the obtained data can be managed by electronic data processing. Donors were regarded as IgA-deficient when their serum level was below 10 mg/dl (normal values 90 to 450 mg/dl). At this concentration ELISA shows acceptable reproducibility (within +/- 15% limits). We found 8 deficient donors, i.e. one in 382 or 0.26%. Seven of these donors were totally deficient by ELISA, 2 of them having a strong anti-IgA in their serum. We conclude that ELISA is a simple, reliable, and inexpensive method for screening blood donors for IgA-deficiency.

摘要

本文描述了一种借助酶联免疫吸附测定法（ELISA）对献血者进行大规模IgA缺乏筛查的方案。ELISA方法被证明快速可靠，所获得的数据可用电子数据处理进行管理。当献血者血清水平低于10mg/dl（正常值90至450mg/dl）时，被视为IgA缺乏。在此浓度下，ELISA显示出可接受的重复性（在±15%范围内）。我们发现8名缺乏者，即每382人中就有1人，占0.26%。其中7名献血者经ELISA检测完全缺乏，其中2人血清中有强效抗IgA。我们得出结论，ELISA是一种用于筛查献血者IgA缺乏的简单、可靠且廉价的方法。