Hunt A F, Allen D L, Aries D L, Strange J J
Vox Sang. 1985;48(2):84-8. doi: 10.1111/j.1423-0410.1985.tb00151.x.
A protocol for large-scale screening of blood donors for IgA deficiency using a tanned cell passive haemagglutination-inhibition (PHI) technique and enzyme-linked immunosorbent assay (ELISA) is described. Evidence presented suggests that native serum from a case of IgA paraproteinaemia, suitably diluted and heated to 56 degrees C for 60 min, can act as a satisfactory and simple alternative to purified IgA preparations in the PHI test. When PHI and ELISA techniques were used in combination to screen 41,851 random donors, 58 were found to contain less than 200 ng/ml of IgA and were classified as totally IgA-deficient (1:721). This is in close agreement with the data from other workers.
本文描述了一种使用鞣酸细胞被动血凝抑制（PHI）技术和酶联免疫吸附测定（ELISA）对献血者进行大规模IgA缺乏筛查的方案。所提供的证据表明，来自一例IgA副蛋白血症患者的天然血清，经过适当稀释并加热至56℃ 60分钟后，可作为PHI试验中纯化IgA制剂的一种令人满意且简单的替代品。当使用PHI和ELISA技术联合筛查41851名随机献血者时，发现58人IgA含量低于200 ng/ml，被归类为完全IgA缺乏（1:721）。这与其他研究人员的数据密切一致。
