Palmer R J, Holland G R
Department of Restorative Dentistry, Faculty of Dentistry, University of Alberta, Edmonton, Canada.
J Anat. 1988 Apr;157:163-8.
The aim of this study was to determine if measuring trigeminal ganglion neuron profiles containing nucleoli in histological sections gave a reasonable estimation of cell size. Serial sections of ferret trigeminal ganglia were prepared, and neuron profiles traced with the aid of a camera lucida. The cross sectional area of the profiles was measured. Profiles containing nucleoli formed the upper part of the size spectrum and were significantly larger than the profiles that did not contain nucleoli. When serial profiles of individual neurons were examined the largest profile contained the nucleolus in 20 of 27 cases, and the mean sizes of 'largest' and 'nucleolus-containing' profiles did not differ significantly. Computer reconstructions of individual cells show the nucleolus to be central in almost all cells viewed from almost every projection. It is concluded that the nucleolus lies at, or very near to, the centre of the neuron and at its widest dimension. Measuring the size of profiles containing nucleoli gives a satisfactory representation of cell size.
本研究的目的是确定在组织学切片中测量包含核仁的三叉神经节神经元轮廓是否能合理估计细胞大小。制备了雪貂三叉神经节的连续切片，并借助明视野显微镜描绘出神经元轮廓。测量轮廓的横截面积。包含核仁的轮廓构成了大小谱的上部，并且明显大于不包含核仁的轮廓。当检查单个神经元的连续轮廓时，在27例中的20例中，最大的轮廓包含核仁，并且“最大”和“含核仁”轮廓的平均大小没有显著差异。单个细胞的计算机重建显示，从几乎每个投影方向观察，几乎所有细胞中的核仁都位于中央。得出的结论是，核仁位于神经元的中心或非常靠近中心的位置，并且处于其最宽的尺寸处。测量包含核仁的轮廓的大小能令人满意地反映细胞大小。
