Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5000-801, Vila Real, Portugal.
Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal.
Parasitol Res. 2020 Sep;119(9):3109-3112. doi: 10.1007/s00436-020-06794-1. Epub 2020 Jul 9.
Recent studies have described Spirocerca lupi-like nematodes in the stomach of red foxes (Vulpes vulpes) in Europe. A phylogenetic analysis of those specimens using mitochondrial DNA and their morphological reexamination allowed their characterization as a different species, Spirocerca vulpis. Between the years of 2010 and 2017, roundworms were collected from seven red foxes of northeastern Portugal found at necropsy with nodular lesions on their stomach wall. Histopathological analysis of four foxes revealed granulomatous lesions of the gastric nodules. On morphological assessment, by light microscopy, nematodes revealed the presence of six triangular teeth-like buccal capsule structures, which are absent in S. lupi. Polymerase chain reaction was run to amplify a 551 bp partial fragment of the cytochrome c oxidase subunit 1 gene. Sequences were 99% similar to S. vulpis (85% coverage) of red foxes from Spain and Bosnia and Herzegovina, 99% similar (99% coverage) to sequences of Spirocerca sp. of red foxes from Denmark and 93% similar (99% coverage) to S. lupi from South Africa. This is the first report of S. vulpis in foxes or any other host from Portugal.
最近的研究描述了在欧洲红狐（Vulpes vulpes）胃中的类似旋毛虫属线虫。对这些样本进行的线粒体 DNA 系统发育分析及其形态学再检查，将其鉴定为不同的物种，即狐旋毛虫（Spirocerca vulpis）。2010 年至 2017 年间，在葡萄牙东北部的七只死于尸检的红狐的胃壁结节处采集了圆线虫。对四只狐狸的组织病理学分析显示胃结节有肉芽肿病变。通过光镜形态评估，线虫显示存在六个三角形的牙齿状口囊结构，这在旋毛虫属中不存在。进行聚合酶链式反应以扩增细胞色素 c 氧化酶亚基 1 基因的 551bp 部分片段。序列与西班牙和波斯尼亚和黑塞哥维那的红狐中的狐旋毛虫（99%相似，85%覆盖率），丹麦的红狐中的旋毛虫属（99%相似，99%覆盖率）以及南非的旋毛虫属（93%相似，99%覆盖率）的序列 99%相似。这是葡萄牙首次报道狐旋毛虫在狐狸或任何其他宿主中的存在。
