Leão Aloma Eiterer, Coelho Sandra Gesteira, Azevedo Rafael Alves, Campos Mariana Magalhães, Machado Fernanda Samarini, Laguna Juliana Guimarães, Ferreira Alexandre Lima, Pereira Luiz Gustavo Ribeiro, Tomich Thierry Ribeiro, de Fátima Costa Suely, Machado Marco Antônio, de Lima Reis Daniele Ribeiro
Department of Animal Science, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
EMBRAPA - Dairy Cattle Research Center, Juiz de Fora, Minas Gerais, Brazil.
PLoS One. 2020 Jul 9;15(7):e0234610. doi: 10.1371/journal.pone.0234610. eCollection 2020.
The objective of this study was to evaluate the effect of the physical form of starter and inclusion of hay in the diet of preweaning dairy calves on performance, digestibility, ruminal development, and mRNA expression of genes involved in ruminal metabolism. Holstein × Gyr crossbred male calves (n = 38 1day old) were assigned to 3 treatments for 9 weeks: Control (n = 13; pellet starter with 4 mm diameter and 18 mm length and 4% steam-flaked corn), Ground (n = 12; same starter of the control but ground pass through a 4.0 mm sieve), or Ground plus 5% chopped Tifton hay GH (n = 13). All calves were fed 4 L/d of whole milk up to 63 d of age and were abruptly weaned at 64 d of age. Water and diets were offered ad libitum. Samples of ruminal contents were obtained from all animals at 30, 45, and 60 d of age to evaluate pH, ammonia nitrogen, and volatile fatty acids (VFA). At 55 d of age, an apparent digestibility assay was performed using 18 animals (n = 6/ treatment). At 65 d of age, the 18 animals were euthanized to evaluate the development of the digestive tract. The physical form of starter and the dietary inclusion of hay did not influence starter intake (Control 326 g/d, Ground 314 g/d and GH 365 g/d), daily weight gain (Control 541g/d, Ground 531g/d and GH 606g/d), feed efficiency, apparent nutrient digestibility, energy partitioning, nitrogen balance, ruminal pH, ammonia nitrogen concentration, VFA, the development of the digestive tract and the mRNA expression of genes involved in AGV metabolism.
本研究的目的是评估断奶前犊牛日粮中开食料的物理形态以及干草添加对其生产性能、消化率、瘤胃发育以及瘤胃代谢相关基因mRNA表达的影响。将荷斯坦×吉尔杂交雄性犊牛(n = 38,1日龄)分为3组,进行为期9周的试验:对照组(n = 13;直径4 mm、长度18 mm的颗粒开食料和4%的蒸汽压片玉米)、粉碎组(n = 12;与对照组相同的开食料但经过4.0 mm筛网粉碎)或粉碎+5%切碎的蒂夫顿干草组(GH组,n = 13)。所有犊牛在63日龄前每天饲喂4 L全脂牛奶,并在64日龄时突然断奶。自由提供水和日粮。在30、45和60日龄时从所有动物采集瘤胃内容物样本,以评估pH值、氨氮和挥发性脂肪酸(VFA)。在55日龄时,使用18头动物(每组n = 6)进行表观消化率测定。在65日龄时,对这18头动物实施安乐死以评估消化道发育情况。开食料的物理形态和日粮中干草的添加对开食料采食量(对照组326 g/d、粉碎组314 g/d和GH组365 g/d)、日增重(对照组541 g/d、粉碎组531 g/d和GH组606 g/d)、饲料效率、表观养分消化率、能量分配、氮平衡、瘤胃pH值、氨氮浓度、VFA、消化道发育以及AGV代谢相关基因的mRNA表达均无影响。