• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转录组测序显示，敲低FOXL2会影响鸡排卵前卵泡细胞的细胞增殖、细胞周期和DNA复制。

Transcriptome sequencing revealed that knocking down FOXL2 affected cell proliferation, the cell cycle, and DNA replication in chicken pre-ovulatory follicle cells.

作者信息

Luo Wei, Gu Lantao, Li Jinqiu, Gong Yanzhang

机构信息

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.

Guilin Medical University, Guilin, Guangxi, China.

出版信息

PLoS One. 2020 Jul 9;15(7):e0234795. doi: 10.1371/journal.pone.0234795. eCollection 2020.

DOI:10.1371/journal.pone.0234795
PMID:32645018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7347172/
Abstract

Forkhead box L2 (FOXL2) is a single-exon gene encoding a forkhead transcription factor, which is mainly expressed in the ovary, eyelids and the pituitary gland. FOXL2 plays an essential role in ovarian development. To reveal the effects of FOXL2 on the biological process and gene expression of ovarian granulosa cells (GCs), we established stable FOXL2-knockdown GCs and then analysed them using transcriptome sequencing. It was observed that knocking down FOXL2 affected the biological processes of cell proliferation, DNA replication, and apoptosis and affected cell cycle progression. FOXL2 knockdown promoted cell proliferation and DNA replication, decreased cell apoptosis, and promoted mitosis. In addition, by comparing the transcriptome after FOXL2 knockdown, we found a series of DEGs (differentially expressed genes) and related pathways. These results indicated that, through mediating these genes and pathways, the FOXL2 might induce the cell proliferation, cycle, and DNA replication, and play a key role during ovarian development and maintenance.

摘要

叉头框L2（FOXL2）是一个单外显子基因，编码一种叉头转录因子，主要在卵巢、眼睑和垂体中表达。FOXL2在卵巢发育中起重要作用。为了揭示FOXL2对卵巢颗粒细胞（GCs）生物学过程和基因表达的影响，我们建立了稳定敲低FOXL2的GCs，然后使用转录组测序对其进行分析。观察到敲低FOXL2影响细胞增殖、DNA复制和凋亡的生物学过程，并影响细胞周期进程。敲低FOXL2促进细胞增殖和DNA复制，减少细胞凋亡，并促进有丝分裂。此外，通过比较敲低FOXL2后的转录组，我们发现了一系列差异表达基因（DEGs）和相关途径。这些结果表明，FOXL2可能通过介导这些基因和途径诱导细胞增殖、周期和DNA复制，并在卵巢发育和维持过程中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/8ce3f3640fec/pone.0234795.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/32f761e35977/pone.0234795.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/df1dddac9051/pone.0234795.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/95a5ebbbbcd8/pone.0234795.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/432f3cea35bf/pone.0234795.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/480341f84356/pone.0234795.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/89c9eb5c655f/pone.0234795.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/4c11dd054e50/pone.0234795.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/a7bd552d50d1/pone.0234795.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/8ce3f3640fec/pone.0234795.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/32f761e35977/pone.0234795.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/df1dddac9051/pone.0234795.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/95a5ebbbbcd8/pone.0234795.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/432f3cea35bf/pone.0234795.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/480341f84356/pone.0234795.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/89c9eb5c655f/pone.0234795.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/4c11dd054e50/pone.0234795.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/a7bd552d50d1/pone.0234795.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c038/7347172/8ce3f3640fec/pone.0234795.g009.jpg

相似文献

1
Transcriptome sequencing revealed that knocking down FOXL2 affected cell proliferation, the cell cycle, and DNA replication in chicken pre-ovulatory follicle cells.转录组测序显示，敲低FOXL2会影响鸡排卵前卵泡细胞的细胞增殖、细胞周期和DNA复制。
PLoS One. 2020 Jul 9;15(7):e0234795. doi: 10.1371/journal.pone.0234795. eCollection 2020.
2
Transcriptome analysis of the potential roles of FOXL2 in chicken pre-hierarchical and pre-ovulatory granulosa cells.FOXL2在鸡等级前和排卵前颗粒细胞中潜在作用的转录组分析
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Mar;21:56-66. doi: 10.1016/j.cbd.2016.12.003. Epub 2017 Jan 5.
3
FOXL2 regulates RhoA expression to change actin cytoskeleton rearrangement in granulosa cells of chicken pre-ovulatory follicles†.FOXL2 通过调节 RhoA 的表达来改变鸡排卵前卵泡颗粒细胞中细胞骨架的重排。
Biol Reprod. 2024 Aug 15;111(2):391-405. doi: 10.1093/biolre/ioae082.
4
Aberrant and constitutive expression of FOXL2 impairs ovarian development and functions in mice.FOXL2 的异常和组成型表达会损害小鼠的卵巢发育和功能。
Biol Reprod. 2020 Oct 29;103(5):966-977. doi: 10.1093/biolre/ioaa146.
5
Minireview: roles of the forkhead transcription factor FOXL2 in granulosa cell biology and pathology.综述：叉头框转录因子 FOXL2 在颗粒细胞生物学和病理学中的作用。
Endocrinology. 2011 Apr;152(4):1199-208. doi: 10.1210/en.2010-1041. Epub 2011 Jan 19.
6
FOXL2: a central transcription factor of the ovary.FOXL2：卵巢的核心转录因子。
J Mol Endocrinol. 2013 Dec 19;52(1):R17-33. doi: 10.1530/JME-13-0159. Print 2014 Feb.
7
Mouse forkhead L2 maintains repression of FSH-dependent genes in the granulosa cell.鼠叉头框 L2 维持颗粒细胞中 FSH 依赖基因的抑制。
Reproduction. 2012 Oct;144(4):485-94. doi: 10.1530/REP-11-0259. Epub 2012 Jul 30.
8
FOXL2 regulates the expression of the Col4a1 collagen gene in chicken granulosa cells.FOXL2 调控鸡颗粒细胞中 Col4a1 胶原基因的表达。
Mol Reprod Dev. 2022 Feb;89(2):95-103. doi: 10.1002/mrd.23554. Epub 2022 Feb 4.
9
Cooperative Effects of FOXL2 with the Members of TGF-β Superfamily on FSH Receptor mRNA Expression and Granulosa Cell Proliferation from Hen Prehierarchical Follicles.FOXL2与转化生长因子-β超家族成员对母鸡等级前卵泡FSH受体mRNA表达及颗粒细胞增殖的协同作用
PLoS One. 2015 Oct 23;10(10):e0141062. doi: 10.1371/journal.pone.0141062. eCollection 2015.
10
Estrogen regulates forkhead transcription factor 2 to promote apoptosis of human ovarian granulosa-like tumor cells.雌激素调节叉头转录因子 2 促进人卵巢颗粒细胞瘤细胞凋亡。
J Steroid Biochem Mol Biol. 2019 Nov;194:105418. doi: 10.1016/j.jsbmb.2019.105418. Epub 2019 Jul 31.

引用本文的文献

1
Comparative transcriptome analysis reveals involvement of FOXL2 in regulation of ovulation cycle in chicken.比较转录组分析揭示FOXL2参与鸡排卵周期的调控。
Poult Sci. 2025 Jun 19;104(10):105461. doi: 10.1016/j.psj.2025.105461.
2
Overview of chicken embryo genes related to sex differentiation.鸡胚性别分化相关基因概述。
PeerJ. 2024 Mar 19;12:e17072. doi: 10.7717/peerj.17072. eCollection 2024.
3
Overview of Avian Sex Reversal.鸟类性别反转概述。

本文引用的文献

1
Growth differentiation factor 9 promotes follicle-stimulating hormone-induced progesterone production in chicken follicular granulosa cells.生长分化因子9促进鸡卵泡颗粒细胞中促卵泡激素诱导的孕酮生成。
Gen Comp Endocrinol. 2019 May 15;276:69-76. doi: 10.1016/j.ygcen.2019.03.005. Epub 2019 Mar 6.
2
Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka.Cdk9 介导的核孕激素受体磷酸化对于 Medaka 排卵所必需的蛋白酶 Mmp15 的表达是必需的。
Cells. 2019 Mar 4;8(3):215. doi: 10.3390/cells8030215.
3
The switch from cAMP-independent to cAMP-dependent arrest of meiotic prophase is associated with coordinated GPR3 and CDK1 expression in mouse oocytes.
Int J Mol Sci. 2023 May 5;24(9):8284. doi: 10.3390/ijms24098284.
4
Genome-wide identification, evolution and expression analysis of bone morphogenetic protein (BMP) gene family in chinese soft-shell turtle ().中华鳖骨形态发生蛋白（BMP）基因家族的全基因组鉴定、进化及表达分析
Front Genet. 2023 Feb 1;14:1109478. doi: 10.3389/fgene.2023.1109478. eCollection 2023.
5
Single-Cell Atlas of the Chinese Tongue Sole Ovary Reveals Transcriptional Programs of Oogenesis in Fish.中国舌鳎卵巢单细胞图谱揭示鱼类卵子发生的转录程序。
Front Cell Dev Biol. 2022 Mar 1;10:828124. doi: 10.3389/fcell.2022.828124. eCollection 2022.
6
Comparative Analysis Among Different Species Reveals That the Androgen Receptor Regulates Chicken Follicle Selection Through Species-Specific Genes Related to Follicle Development.不同物种间的比较分析表明，雄激素受体通过与卵泡发育相关的物种特异性基因调控鸡卵泡的选择。
Front Genet. 2022 Jan 3;12:752976. doi: 10.3389/fgene.2021.752976. eCollection 2021.
7
Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle.母鸡排卵周期中颗粒细胞长链非编码RNA和信使核糖核酸的系统分析
Animals (Basel). 2021 May 25;11(6):1533. doi: 10.3390/ani11061533.
8
Insights into the pathogenicity of missense variants in the forkhead domain of FOX proteins underlying Mendelian disorders.探讨孟德尔疾病中 FOX 蛋白的叉头结构域错义变异的致病机制。
Hum Genet. 2021 Jul;140(7):999-1010. doi: 10.1007/s00439-021-02267-2. Epub 2021 Feb 27.
从小鼠卵母细胞减数分裂前期不依赖cAMP到依赖cAMP的停滞转变与GPR3和CDK1的协同表达有关。
Dev Biol. 2018 Feb 1;434(1):196-205. doi: 10.1016/j.ydbio.2017.12.014. Epub 2017 Dec 21.
4
FOXL2 Mutation Status in Granulosa Theca Cell Tumors of the Ovary.卵巢颗粒-卵泡膜细胞瘤中的FOXL2突变状态
Int J Gynecol Pathol. 2017 Nov;36(6):568-574. doi: 10.1097/PGP.0000000000000377.
5
Transcriptome analysis of the potential roles of FOXL2 in chicken pre-hierarchical and pre-ovulatory granulosa cells.FOXL2在鸡等级前和排卵前颗粒细胞中潜在作用的转录组分析
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Mar;21:56-66. doi: 10.1016/j.cbd.2016.12.003. Epub 2017 Jan 5.
6
Cooperative Effects of FOXL2 with the Members of TGF-β Superfamily on FSH Receptor mRNA Expression and Granulosa Cell Proliferation from Hen Prehierarchical Follicles.FOXL2与转化生长因子-β超家族成员对母鸡等级前卵泡FSH受体mRNA表达及颗粒细胞增殖的协同作用
PLoS One. 2015 Oct 23;10(10):e0141062. doi: 10.1371/journal.pone.0141062. eCollection 2015.
7
The genetics of premature ovarian failure: current perspectives.卵巢早衰的遗传学：当前观点
Int J Womens Health. 2015 Sep 23;7:799-810. doi: 10.2147/IJWH.S64024. eCollection 2015.
8
Association of novel polymorphisms of forkhead box L2 and growth differentiation factor-9 genes with egg production traits in local Chinese Dagu hens.叉头框L2基因和生长分化因子9基因的新型多态性与中国地方大骨鸡产蛋性状的关联
Poult Sci. 2015 Jan;94(1):88-95. doi: 10.3382/ps/peu023.
9
FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells.FOXL2抑制宫颈癌细胞的增殖、侵袭并促进其凋亡。
Int J Clin Exp Pathol. 2014 Mar 15;7(4):1534-43. eCollection 2014.
10
Signaling by the extracellular matrix protein Reelin promotes granulosa cell proliferation in the chicken follicle.细胞外基质蛋白 Reelin 通过信号转导促进鸡卵泡中的颗粒细胞增殖。
J Biol Chem. 2014 Apr 4;289(14):10182-91. doi: 10.1074/jbc.M113.533489. Epub 2014 Feb 26.