Wang Tie, Xiong Bo, Tan Liping, Yang Youting, Zhang Yue, Ma Mengmeng, Xu Yinghuan, Liao Ling, Sun Guochao, Liang Dong, Xia Hui, Zhang Xiaoai, Wang Zhihui, Wang Jun
College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Funct Plant Biol. 2020 Oct;47(11):977-987. doi: 10.1071/FP20079.
To obtain the compatibility of interstocks crossing with 'Yuanxiaochun', we performed a comparative analysis with five interstocks. From the 60th to 240th day after grafting, there was a significant difference between different treatments. All the new shoot/interstock diameter ratios were <1, indicating that there was no obvious phenomenon of small feet in 'Yuanxiaochun' seedlings of five kinds of interstocks. The density of 'Yuanxiaochun' was significantly different. Chl a, Chl b, T-Chl content of 'Shiranuhi', 'Harumi', 'Tarocco' changed greatly from the 90th to 120th day after grafting. The intercellular CO2 concentration (Ci) of 'Shiranuhi' was significantly higher than the other interstocks. In addition, when 'Yuanxiaochun' was grafted onto 'Shiranuhi', net photosynthetic rate (Pn), stomatal conductance (gs) and tanspiration rate (Tr) were higher. When 'Harumi' were used as the interstocks of 'Yuanxiaochun', the light saturation point (LSP) value was larger, which was conducive to the utilisation of strong light. Moreover, the value of LSP-LCP (LCP, light compensation point) of 'Harumi' and 'Tarocco' were significantly higher than the other three interstocks. The apparent quantum efficiency (AQE), RuBP maximum regeneration rate (Jmax) and maximum carboxylation efficiency of Rubisco (Vcmax) value of 'Shiranuhi' was significantly lower than that of 'Ponkan'. The CO2 compensation point (CCP) of 'Harumi' interstock was lower, but the CO2 saturation point (CSP) of 'Tarocco' interstock was higher than those of other interstocks respectively. There was a small difference in initial fluorescence (F0) of different interstocks. The maximal photochemical efficiency of PSII in the dark (Fv/Fm) of 'Kumquat' was the lowest. In addition, Both Y(II) and ETR values of the 'Yuanxiaochun' leaves of 'Ponkan' interstock was the largest one. However, the non-photochemical quenching (qN) of 'Ponkan' was significantly lower, and that of 'Tarocco' interstock was the highest one. Interstocks have different effect on the growth and development, photosynthetic characteristics related to physiological characteristics of 'Yuanxiaochun' trees. 'Ponkan' and 'Kumquat' as the interstock of 'Yuanxiaochun' was more conducive to the rapid accumulation of photosynthetic products for normal vegetative and reproductive growth of plants.
为获得与‘元小春’砧穗组合的亲和性，我们对5种砧木进行了比较分析。嫁接后第60天至240天，不同处理间存在显著差异。所有新梢/砧木直径比均<1，表明5种砧木的‘元小春’幼苗均无明显的小脚现象。‘元小春’的密度差异显著。‘不知火’‘春见’‘塔罗科’的叶绿素a、叶绿素b、总叶绿素含量在嫁接后第90天至120天变化较大。‘不知火’的胞间CO₂浓度（Ci）显著高于其他砧木。此外，当‘元小春’嫁接到‘不知火’上时，净光合速率（Pn）、气孔导度（gs）和蒸腾速率（Tr）较高。当以‘春见’为‘元小春’的砧木时，光饱和点（LSP）值较大，有利于强光的利用。此外，‘春见’和‘塔罗科’的LSP-LCP（LCP，光补偿点）值显著高于其他3种砧木。‘不知火’的表观量子效率（AQE）、RuBP最大再生速率（Jmax）和Rubisco最大羧化效率（Vcmax）值显著低于‘椪柑’。‘春见’砧木的CO₂补偿点（CCP）较低，但‘塔罗科’砧木的CO₂饱和点（CSP）分别高于其他砧木。不同砧木的初始荧光（F0）差异较小。‘金橘’在黑暗中PSII的最大光化学效率（Fv/Fm）最低。此外，‘椪柑’砧木的‘元小春’叶片的Y（II）和ETR值均为最大。然而，‘椪柑’的非光化学猝灭（qN）显著较低，而‘塔罗科’砧木的qN最高。砧木对‘元小春’树的生长发育、与生理特性相关的光合特性有不同影响。‘椪柑’和‘金橘’作为‘元小春’的砧木更有利于光合产物的快速积累，促进植株正常的营养生长和生殖生长。
