Wang Tie, Deng Lijun, Huang Shengjia, Xiong Bo, Muhammad Ihtisham, Zheng Zhendong, Zheng Wei, Qin Zeyu, Zhang Mingfei, Sun Guochao, Wang Jun, Wang Zhihui
College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.
College of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, China.
Biology (Basel). 2022 Nov 9;11(11):1639. doi: 10.3390/biology11111639.
The interstock, a stock between the rootstock and scion, has a significant regulatory effect on the stock and scion, and its function is highly dependent on graft compatibility. To assess the graft compatibility of the interstock and scion, 'Yuanxiaochun' was top grafted onto 'Ponkan', 'Shiranuhi', 'Harumi', 'Tarocco', and 'Kumquat'. The results showed significant differences in the survival ratio and preservation ratio among different combinations. Grafting compatibility in the early stages of grafting was associated with the genetic relationship. The biomass accumulation revealed that the interstock could influence both the rootstock and the scion. The physiological and biochemical traits analysis suggested that SPAD reading and soluble sugar could be employed as preselected indices to evaluate graft compatibility in the late stage of grafting. These results indicated that the evaluation of graft compatibility was a dynamic process. The findings provided a new approach for studying the stock and scion interaction mechanisms mediated by interstock, and directly provided a theoretical and practical basis for the high-grafting of 'Yuanxiaochun' citrus.
中间砧是位于砧木和接穗之间的一段茎干，对砧木和接穗具有显著的调控作用，其功能高度依赖于嫁接亲和力。为了评估中间砧与接穗的嫁接亲和力，将‘媛小春’高接在‘椪柑’、‘不知火’、‘春见’、‘塔罗科血橙’和‘金橘’上。结果表明，不同组合之间的成活率和保存率存在显著差异。嫁接早期的嫁接亲和力与亲缘关系有关。生物量积累表明，中间砧对砧木和接穗都有影响。生理生化特性分析表明，SPAD值和可溶性糖可作为嫁接后期评估嫁接亲和力的预选指标。这些结果表明，嫁接亲和力的评估是一个动态过程。该研究结果为研究中间砧介导的砧穗互作机制提供了新途径，直接为‘媛小春’柑橘高接换种提供了理论和实践依据。
