Department of Surgery, Keck School of Medicine of USC, Los Angeles, California; Department of Surgery and Biomedical Engineering, Keck School of Medicine USC, Los Angeles, CA.
Department of Surgery, Keck School of Medicine of USC, Los Angeles, California.
Nanomedicine. 2020 Oct;29:102263. doi: 10.1016/j.nano.2020.102263. Epub 2020 Jul 6.
Wound healing has been intensely studied to expedite recovery times and reduce scarring. However, current technologies fail to achieve regenerative capabilities, leaving wounds with scarring and lack of skin accessories. The recent emergence of nanotechnology has provided a new clinical modality of zinc nanoparticles in wound care. This present study investigated Zinc Sulfide nanoparticles (ZnS-NP) on wound healing in vitro with 2D and 3D models and in vivo with rat full-thickness wound model. ZnS-NP inhibited fetal bovine serum-stimulated rat skin fibroblast cell proliferation, altered cytoskeletal organization, and reduced collagen synthesis as well as contractile activity. ZnS-NP regulated redox homeostatsis and promoted fibroblast viability in 3D hypoxia conditions. In the rat full-thickness wound model, ZnS-NP reduced wound contraction, enhanced re-epithelization, and promoted skin appendage formation. The biological activities of ZnS-NPs determined in our current study may suggest promising practical applications for topical or systemic treatment for wound repair.
伤口愈合一直是研究的热点，旨在缩短恢复时间并减少疤痕形成。然而，目前的技术无法实现再生能力，导致伤口留下疤痕和缺乏皮肤附件。最近纳米技术的出现为锌纳米粒子在伤口护理中的应用提供了新的临床模式。本研究通过 2D 和 3D 模型体外和大鼠全层创面模型体内研究了硫化锌纳米粒子（ZnS-NP）对伤口愈合的影响。ZnS-NP 抑制胎牛血清刺激的大鼠皮肤成纤维细胞增殖，改变细胞骨架组织，减少胶原合成和收缩活性。ZnS-NP 调节氧化还原内稳态，并在 3D 低氧条件下促进成纤维细胞活力。在大鼠全层创面模型中，ZnS-NP 减少创面收缩，促进再上皮化，并促进皮肤附属物形成。本研究中确定的 ZnS-NP 的生物学活性表明，ZnS-NP 可能具有作为局部或全身治疗伤口修复的有前途的实际应用。
