Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
Biomolecules. 2024 Aug 22;14(8):1041. doi: 10.3390/biom14081041.
Blood-contact devices are prone to inflammation, endothelial dysfunction, coagulation, and the uncontrolled release of metal ions during implantation and service. Therefore, it is essential to make these multifunctional. Herein, a superhydrophobic DE@ZnS-ZnO@SA film (composed of dabigatran ester, zinc sulfite, zinc oxide, and stearic acid, respectively) is produced. The prepared film has non-adhesion and antibacterial properties, superior mechanical stability, durability, corrosion resistance, and is self-cleaning and blood-repellent. The results of the hemolysis, cytotoxicity, and other anticoagulant experiments revealed that the film had good blood compatibility, no cytotoxicity, and excellent anticoagulant properties. The film displays anticoagulant properties even after being immersed in Phosphate-Buffered Saline (PBS) for 7 days. Furthermore, the film can spontaneously release HS gas for 90 h after soaking in an acidic environment (pH = 6) for 90 h. This property improves the acidic microenvironment of the lesion and promotes the proliferation of endothelial cells by using HS gas. In addition, the film can inhibit the uncontrollable release of Zn ions, avoiding its toxicity even when immersed in an acid environment for 35 days. This time-sequential functionalized surface has the potential to typify the future of blood-contacting scaffolds for long-lasting use.
血液接触装置在植入和使用过程中容易发生炎症、内皮功能障碍、凝血和金属离子的失控释放。因此，使这些装置具有多功能性至关重要。在此，制备了一种超疏水 DE@ZnS-ZnO@SA 薄膜（分别由达比加群酯、亚硫酸锌、氧化锌和硬脂酸组成）。所制备的薄膜具有非粘连和抗菌性能、优异的机械稳定性、耐久性、耐腐蚀性以及自清洁和抗血液附着性。溶血、细胞毒性等抗凝实验结果表明，该薄膜具有良好的血液相容性、无细胞毒性和优异的抗凝性能。即使在磷酸盐缓冲盐水（PBS）中浸泡 7 天后，薄膜仍具有抗凝性能。此外，薄膜在酸性环境（pH = 6）中浸泡 90 h 后，可自发释放 HS 气体 90 h。该特性改善了病变部位的酸性微环境，并通过 HS 气体促进内皮细胞的增殖。此外，该薄膜可以抑制 Zn 离子的不可控释放，即使在酸性环境中浸泡 35 天也能避免其毒性。这种时间序列功能化表面具有成为长期使用的血液接触支架的未来的潜力。
