Han Xin, Liu Zongbin, Ma Yuan, Zhang Kai, Qin Lidong
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, 77030, USA.
Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, 10065, USA.
Adv Biosyst. 2017 Feb;1(1-2):e1600007. doi: 10.1002/adbi.201600007. Epub 2017 Jan 16.
This study reports a microfluidic cell deformation-based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard-to-transfect human primary CD4+ T cells. The RNP based CRISPR-Cas9 system has great advantage in shortening reaction time and reducing off-target problems, which holds great potential in future gene therapy applications.
本研究报道了一种基于微流控细胞变形的方法，用于将Cas9核糖核蛋白（RNP）复合物递送至不同细胞类型以进行高效基因组编辑，包括难以转染的人类原代CD4+ T细胞。基于RNP的CRISPR-Cas9系统在缩短反应时间和减少脱靶问题方面具有巨大优势，在未来基因治疗应用中具有巨大潜力。
