Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Teknik University, Trabzon, Turkey.
Comb Chem High Throughput Screen. 2021;24(3):376-385. doi: 10.2174/1386207323666200709170450.
Electroanalytical methods are very functional to detect drugs in pharmaceuticals (tablets, syrups, suppositories, creams, and ointments) and biological samples.
This study is aimed to make selective, sensitive, simple, fast, and low cost electrochemical analysis of expectorant drug guaifenesin in pharmaceuticals and serum samples.
Differential pulse adsorptive stripping voltammetric method for determination of guaifenesin on a poly(acridine orange) modified glassy carbon electrode has been developed. Glassy carbon electrode was modified with electropolymerization of the acridine orange monomer for the sensitive determination of guaifenesin. Guaifenesin provided highly reproducible and welldefined irreversible oxidation peaks at +1.125 V and +1.128 V (vs. Ag/AgCl) in the selected supporting electrolyte and human serum samples, respectively.
Under optimized conditions, linear response of peak current on the concentration of guaifenesin has been obtained in the ranges of 2.00×10-7 to 1.00×10-4 M in Britton Robinson buffer solution at pH 7.0 and 4.00×10-7 to 1.00×10-4 M in serum samples. The precision of the method was detected by intraday and inter-day repeatability studies in the supporting electrolyte and serum samples media.
The analytical applicability of the proposed method exhibited satisfying determination results for guaifenesin from pharmaceutical dosage forms (syrup) and human serum samples without any pre-separation procedures.
电化学分析方法在检测药物（片剂、糖浆、栓剂、乳膏和软膏）和生物样本中的应用非常广泛。
本研究旨在建立一种选择性、灵敏、简单、快速且低成本的电化学分析方法，用于检测药物和血清样本中的祛痰药愈创甘油醚。
采用聚吖啶橙修饰玻碳电极，建立了差分脉冲吸附溶出伏安法测定愈创甘油醚的方法。通过吖啶橙单体的电化学聚合对玻碳电极进行修饰，实现了对愈创甘油醚的灵敏测定。在所选支持电解质和人血清样品中，愈创甘油醚分别在+1.125 V 和+1.128 V（相对于 Ag/AgCl）处提供了高度重现性和定义明确的不可逆氧化峰。
在优化条件下，在 Britton Robinson 缓冲溶液（pH 7.0）中，峰电流与愈创甘油醚浓度在 2.00×10-7 至 1.00×10-4 M 范围内呈线性关系，在血清样品中，峰电流与愈创甘油醚浓度在 4.00×10-7 至 1.00×10-4 M 范围内呈线性关系。该方法在支持电解质和血清样品介质中的日内和日间重复性研究中检测到了方法的精密度。
该方法在不进行任何预分离处理的情况下，对药物制剂（糖浆）和人血清样本中的愈创甘油醚具有令人满意的测定结果，具有良好的分析适用性。
