Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Systems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkhuntien, Bangkok, Thailand.
BMC Res Notes. 2020 Jul 9;13(1):329. doi: 10.1186/s13104-020-05168-1.
Genome sequences are a vital resource for accelerating the biological exploration of an organism of interest. Pythium destruens (a synonym of Pythium insidiosum) causes a difficult-to-treat infectious disease called pythiosis worldwide. Detection and management of pythiosis are challenging. Basic knowledge of the disease is lacking. Genomes of this organism isolated from different continents (i.e., Asia and the Americas) have been sequenced and publicly available. Here, we sequenced the genome of an Australian isolate of P. destruens. Genome data will facilitate the comparative analysis of this and related species at the molecular level.
Genomic DNA of the P. destruens strain ATCC 64221, isolated from a horse with pythiosis in Australia, was used to prepare one paired-end library (with 180-bp insert) for next-generation sequencing, using the Illumina HiSeq 2500 short-read platform. Raw reads were cleaned and assembled by several bioinformatics tools. A total of 20,860,454 processed reads, accounted for 2,614,890,553 total bases, can be assembled into a 37.8-Mb genome, consisting 13,060 contigs (average length: 2896 bases; range: 300-142,967), N of 11,370 bases, and 2.9% 'N' composition. The genome was determined 85.9% completeness, contained 14,424 predicted genes, and can be retrieved online at the NCBI/DDBJ databases under the accession number BCFQ01000000.1.
基因组序列是加速感兴趣生物体的生物探索的重要资源。腐霉属（同义名：潜隐腐霉）引起一种全球性的、难以治疗的传染性疾病，称为腐霉病。腐霉病的检测和管理具有挑战性，对这种疾病的基本认识也很缺乏。该生物体的基因组已从不同大陆（即亚洲和美洲）分离并测序，且已公开。在此，我们对一株来自澳大利亚的腐霉属分离株进行了基因组测序。基因组数据将有助于在分子水平上对该分离株和相关物种进行比较分析。
从澳大利亚一匹患有腐霉病的马中分离到的腐霉属菌株 ATCC 64221 的基因组 DNA 用于制备一个用于下一代测序的配对末端文库（插入片段长 180bp），使用 Illumina HiSeq 2500 短读平台。原始读取经过多个生物信息学工具进行清理和组装。共获得 20860454 条处理后的读取，占总碱基 2614890533 个，可组装成一个 37.8Mb 的基因组，由 13060 个 contigs（平均长度：2896 个碱基；范围：300-142967）、N 为 11370 个碱基和 2.9%的“N”组成。基因组的完整性为 85.9%，包含 14424 个预测基因，可在 NCBI/DDBJ 数据库中以注册号 BCFQ01000000.1 在线检索。
