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通过多重聚合酶链反应、DNA条形码和蛋白质组学分析对从泰国城乡地区分离出的[具体对象未明确]进行鉴定和生物分型。

Identification and Biotyping of Isolated from Urban and Rural Areas of Thailand by Multiplex PCR, DNA Barcode, and Proteomic Analyses.

作者信息

Mar Htun Zin, Laikul Aree, Pathomsakulwong Watcharapol, Yurayart Chompoonek, Lohnoo Tassanee, Yingyong Wanta, Kumsang Yothin, Payattikul Penpan, Sae-Chew Pattarana, Rujirawat Thidarat, Jittorntam Paisan, Jaturapaktrarak Chalisa, Chongtrakool Piriyaporn, Krajaejun Theerapong

机构信息

Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

出版信息

J Fungi (Basel). 2021 Mar 24;7(4):242. doi: 10.3390/jof7040242.

DOI:10.3390/jof7040242
PMID:33804838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8063814/
Abstract

causes pythiosis, a fatal infectious disease of humans and animals worldwide. Prompt diagnosis and treatment are essential to improve the clinical outcome of pythiosis. Diagnosis of relies on immunological, molecular, and proteomic assays. The main treatment of pythiosis aims to surgically remove all affected tissue to prevent recurrent infection. Due to the marked increase in case reports, pythiosis has become a public health concern. Thailand is an endemic area of human pythiosis. To obtain a complete picture of how the pathogen circulates in the environment, we surveyed the presence of in urban (Bangkok) and rural areas of Thailand. We employed the hair-baiting technique to screen for in 500 water samples. Twenty-seven culture-positive samples were identified as by multiplex PCR, multi-DNA barcode (rDNA, 1, 2), and mass spectrometric analyses. These environmental strains of fell into Clade-II and -III genotypes and exhibited a close phylogenetic/proteomic relationship with Thai clinical strains. Biodiversity of the environmental strains also existed in a local habitat. In conclusion, is widespread in Thailand. A better understanding of the ecological niche of could lead to the effective prevention and control of this pathogen.

摘要

导致芽生菌病，这是一种在全球范围内对人类和动物都具有致命性的传染病。及时诊断和治疗对于改善芽生菌病的临床结果至关重要。芽生菌病的诊断依赖于免疫学、分子学和蛋白质组学检测。芽生菌病的主要治疗方法旨在通过手术切除所有受影响的组织以防止反复感染。由于病例报告显著增加，芽生菌病已成为一个公共卫生问题。泰国是人类芽生菌病的流行地区。为全面了解该病原体在环境中的传播情况，我们对泰国城市（曼谷）和农村地区的[具体病原体名称未给出]存在情况进行了调查。我们采用毛发诱饵技术对500份水样进行[具体病原体名称未给出]筛查。通过多重PCR、多DNA条形码（rDNA、1、2）和质谱分析，27份培养阳性样本被鉴定为[具体病原体名称未给出]。这些环境中的[具体病原体名称未给出]菌株属于进化枝II和III基因型，并且与泰国临床菌株表现出密切的系统发育/蛋白质组学关系。环境菌株的生物多样性也存在于当地栖息地。总之，[具体病原体名称未给出]在泰国广泛存在。更好地了解[具体病原体名称未给出]的生态位可能会导致对这种病原体的有效预防和控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/05344f877102/jof-07-00242-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/16b369b4dac6/jof-07-00242-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/33e61418e18f/jof-07-00242-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/073464ebb97f/jof-07-00242-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/ee9ed2d39a23/jof-07-00242-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/05344f877102/jof-07-00242-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/16b369b4dac6/jof-07-00242-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/33e61418e18f/jof-07-00242-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/073464ebb97f/jof-07-00242-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/ee9ed2d39a23/jof-07-00242-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/992c/8063814/05344f877102/jof-07-00242-g005.jpg

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