Department of Microbiology, P.S.G. College of Arts and Science, Coimbatore 641 014, Tamil Nadu, India.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
J Infect Public Health. 2020 Oct;13(10):1522-1532. doi: 10.1016/j.jiph.2020.06.025. Epub 2020 Jul 6.
Actinomycetes show an active role in microbial disease control and antimicrobial metabolism production and were shown the greatest potentialities as sources of antimicrobial agents. The past few decades, an extensive literature has accumulated on the production of bioactive compounds from actinomycetes, particularily genus Streptomyces.
The actinomycetes were isolated with starch casein nitrate (SCN) agar medium. The prospective isolate was subjected to antimicrobial metabolites production. Further, the bio-extract was evaluated their biological properites by agar well diffusion assay and finally the extract was anaylized through GC-MS.
In the present study, isolated 9 actinomycetes and the isolates were examined for their antifungal activities. Of these nine isolates, the isolate MK_MSt033 picked out from the rest, hences, it showed significant control towards the selected microbial pathogens. The prospective strain MK_MSt033 was determined as Nocardiopsis sp. The strain displayed effective antimicrobial activities against both bacterial and fungal pathgoens such as Escherichia coli, Staphylococcus aureus, Pencillium sp., and Aspergillus flavus respectively. Subsequently the chemical nature of the compounds produced by the potential isolate MK_MSt033 and it was successfully determined by GC-MS and it were 18 compounds with different retention time.
The identified isolate Nocardiopsis sp. MK_MSt033 exhibited potential antimicrobial activities against selected microbial pathogens. Thus, the soil inhabiting Nocardiopsis sp. has explored for pharmaceutically active compounds with promising medical applications.
放线菌在微生物疾病控制和抗菌代谢产物的产生中发挥积极作用，被认为是抗菌药物的最有潜力的来源。过去几十年，大量文献积累了放线菌产生生物活性化合物的研究，特别是链霉菌属。
采用淀粉酪蛋白硝酸盐（SCN）琼脂培养基分离放线菌。对潜在的分离物进行抗菌代谢产物的产生。进一步，通过琼脂孔扩散法评估生物提取物的生物特性，最后通过 GC-MS 对提取物进行分析。
在本研究中，分离出 9 株放线菌，并对其进行了抗真菌活性的检测。在这 9 株分离物中，从其余分离物中挑选出的 MK_MSt033 分离物表现出对所选微生物病原体的显著控制作用。该潜在菌株 MK_MSt033 被鉴定为诺卡氏菌属。该菌株对细菌和真菌病原体均表现出有效的抗菌活性，如大肠杆菌、金黄色葡萄球菌、青霉属和黄曲霉属等。随后，通过 GC-MS 成功确定了潜在分离物 MK_MSt033 产生的化合物的化学性质，共有 18 种具有不同保留时间的化合物。
鉴定出的诺卡氏菌属 MK_MSt033 对选定的微生物病原体表现出潜在的抗菌活性。因此，土壤栖息的诺卡氏菌属被探索用于具有有前途的医疗应用的药物活性化合物。
