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Papaya ringspot virus Populations From East Timorese and Northern Australian Cucurbit Crops: Biological and Molecular Properties, and Absence of Genetic Connectivity.来自东帝汶和澳大利亚北部葫芦科作物的木瓜环斑病毒种群：生物学和分子特性，以及缺乏遗传连通性
Plant Dis. 2017 Jun;101(6):985-993. doi: 10.1094/PDIS-10-16-1499-RE. Epub 2017 Apr 4.
2
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Genome Res. 2017 May;27(5):824-834. doi: 10.1101/gr.213959.116. Epub 2017 Mar 15.
3
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.Geneious Basic：一个集成和可扩展的桌面软件平台，用于组织和分析序列数据。
Bioinformatics. 2012 Jun 15;28(12):1647-9. doi: 10.1093/bioinformatics/bts199. Epub 2012 Apr 27.
4
Translation of tobacco rattle virus RNAs in vitro: four proteins from three RNAs.烟草脆裂病毒RNA的体外翻译：三种RNA产生四种蛋白质。
Virology. 1979 Sep;97(2):256-65. doi: 10.1016/0042-6822(79)90337-4.
5
MUSCLE: a multiple sequence alignment method with reduced time and space complexity.MUSCLE：一种时间和空间复杂度降低的多序列比对方法。
BMC Bioinformatics. 2004 Aug 19;5:113. doi: 10.1186/1471-2105-5-113.
6
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
7
Serotypes and particle dimensions of tobacco rattle viruses from Europe and America.来自欧洲和美洲的烟草脆裂病毒的血清型和颗粒尺寸
Virology. 1966 Apr;28(4):610-20. doi: 10.1016/0042-6822(66)90246-7.
8
Pea early browning virus RNA1 encodes four polypeptides including a putative zinc-finger protein.豌豆早褐病毒RNA1编码四种多肽，包括一种假定的锌指蛋白。
Nucleic Acids Res. 1989 Mar 25;17(6):2245-60. doi: 10.1093/nar/17.6.2245.

转录组测序揭示了一份29岁蚕豆样本的基因组序列。

Transcriptome Sequencing Reveals the Genome Sequence of from a 29-Year-Old Faba Bean Sample.

作者信息

Maina S, Zheng L, King S, McQueen V L, Norton S L, Rodoni B

机构信息

Australian Grains Genebank, Agriculture Victoria, Horsham, Victoria, Australia

Agriculture Victoria, AgriBio, Melbourne, Victoria, Australia.

出版信息

Microbiol Resour Announc. 2020 Jul 9;9(28):e00673-20. doi: 10.1128/MRA.00673-20.

DOI:10.1128/MRA.00673-20
PMID:32646910
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7348028/
Abstract

(PEBV) is transmitted by soil-inhabiting trichodorid nematodes and via seeds. The transcriptome sequencing method, followed by assembly, revealed the PEBV Libyan isolate LyV66-91 genome. Its RNA1 resembled that of UK isolate SP5 with 93.91% nucleotide identity, and its RNA2 had 63.32% nucleotide identity to that of Dutch isolate E116.

摘要

豌豆早褐病毒（PEBV）通过栖息于土壤中的毛刺线虫传播，并通过种子传播。转录组测序方法，随后进行组装，揭示了PEBV利比亚分离株LyV66 - 91的基因组。其RNA1与英国分离株SP5的RNA1相似，核苷酸同一性为93.91%，其RNA2与荷兰分离株E116的RNA2的核苷酸同一性为63.32%。