Gharsallah Charfeddine, Gharsallah Chouchane Sonia, Werghi Sirine, Mehrez Marwa, Fakhfakh Hatem, Gorsane Faten
Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences of Tunis, University of Tunis ElManar, 2092 Tunis, Tunisia.
Higher Institute of Biotechnology, University of Manouba, 2020 Sidi Thabet, Tunisia.
Physiol Mol Biol Plants. 2020 Jul;26(7):1411-1424. doi: 10.1007/s12298-020-00835-w. Epub 2020 Jun 17.
Tomato yellow leaf curl disease (TYLCD) and salinity stress adversely affect tomato production worldwide by causing extensive damages. In Tunisia, identifying TYLCD resistant cultivars selected in different environments is useful to devise counter-measures. To this end, 20 tomato commercial cultivars were screened for different gene alleles' combinations and evaluated either for TYLCD incidence or salinity constraint. We built a biological multi-layer network for integrating, visualizing and modelling generated data. It is a simple representation view linking allelic combinations to tomato cultivars behaviour under viral and salt stresses. In addition, we analyzed differential expression of transcriptions factors (TFs) belonging to WRKY and ERF families in selected resistant (R) and susceptible (S) tomato cultivars. Gene expression was evaluated for short- and long stress exposure to either TYLCSV infection or to both viral and salinity stresses. Evidence is that TFs promote resistance to abiotic and biotic stresses through a complex regulatory network.
番茄黄化曲叶病（TYLCD）和盐胁迫通过造成广泛损害，对全球番茄生产产生不利影响。在突尼斯，鉴定在不同环境中选育出的抗TYLCD品种，有助于制定应对措施。为此，对20个番茄商业品种进行了不同基因等位基因组合的筛选，并对TYLCD发病率或盐胁迫进行了评估。我们构建了一个生物多层网络，用于整合、可视化和模拟生成的数据。它是一个简单的表示视图，将等位基因组合与番茄品种在病毒和盐胁迫下的行为联系起来。此外，我们分析了属于WRKY和ERF家族的转录因子（TFs）在选定的抗性（R）和易感（S）番茄品种中的差异表达。评估了短期和长期暴露于番茄黄化曲叶病毒（TYLCSV）感染或病毒和盐胁迫下的基因表达。有证据表明，转录因子通过复杂的调控网络促进对非生物和生物胁迫的抗性。
