Xie Mengwei, Hu Chunlan, Li Delin, Li Shifeng
Department of Cardiology, Guihang Guiyang Hospital, Guizhou, China.
Dose Response. 2020 Jun 30;18(2):1559325820936124. doi: 10.1177/1559325820936124. eCollection 2020 Apr-Jun.
miR-377 is closely related to myocardial regeneration. miR-377-adjusted mesenchymal stem cells abducted ischemic cardiac angiogenesis. Nevertheless, there were rarely reports about the impact of miR-377 on myocardial ischemia injury. The purpose of this work is that whether miR-377 can protect against myocardial injury caused by hypoxia/reoxygenation (H/R).
Gene expression omnibus database (http://www.ncbi.nlm.nih.gov/geo/; no. GSE53211) was utilized to study the differential expression of miR-377 in patients with an acute ST-segment elevation myocardial infarction and healthy controls. The luciferase activity was determined utilizing the dual-luciferase reporter system. Quantitative real-time polymerase chain reaction and Western blotting were used to measure the messenger RNA and protein level.
Low expression of miR-377 and high expression of leukocyte immunoglobulin-like receptor B2 (LILRB2) were identified in patients with myocardial infarction from analyzing the Gene Expression Omnibus data set. Besides, miR-377 expression was downregulated in cardiomyocyte exposed to H/R. Additionally, overexpression of miR-377 could visibly improve cardiomyocyte injury by regulating cell activity and apoptosis.
In short, our findings suggested that miR-377/LILRB2 might regard as a hopeful therapeutic target for myocardial ischemic.
miR-377与心肌再生密切相关。经miR-377调节的间充质干细胞促进了缺血心肌的血管生成。然而，关于miR-377对心肌缺血损伤影响的报道很少。本研究的目的是探讨miR-377是否能保护心肌免受缺氧/复氧（H/R）引起的损伤。
通过分析基因表达综合数据集，在心肌梗死患者中发现miR-377低表达和白细胞免疫球蛋白样受体B2（LILRB2）高表达。此外，在暴露于H/R的心肌细胞中miR-377表达下调。此外，miR-377的过表达可通过调节细胞活性和凋亡明显改善心肌细胞损伤。
简而言之，我们的研究结果表明，miR-377/LILRB2可能被视为心肌缺血的一个有希望的治疗靶点。
