The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, 5 Weiyang Xi Road, Qindu District, Xianyang, Shaanxi 712000, China.
J Healthc Eng. 2023 Jan 17;2023:7438567. doi: 10.1155/2023/7438567. eCollection 2023.
Hui Yang Jiu Ji (HYJJ) decoction has been applied as a prescription of traditional Chinese medicine for the treatment of chronic heart failure (CHF). However, its comprehensive molecular mechanism remains unclear now. Our study aimed to explore the possible function and lncRNA-miRNA regulation networks of HYJJ on CHF induced by doxorubicin (DOX) in rats. Our study showed that HYJJ could recover cardiac function and alleviate myocardial injury of DOX-induced CHF. Besides, HYJJ had an effect on restraining myocardial apoptosis in CHF rats. Moreover, RNA-sequencing and bioinformatics analysis indicated that among a total of 548 significantly up- and down-regulated differentially expressed (DE) long noncoding RNA (lncRNA), 511 up- and down-regulated DE miRNAs were identified. Cushing's syndrome and Adrenergic signaling in cardiomyocytes were common pathways between DE-lncRNAs-enriched pathways and DE-miRNAs-enriched pathways. Finally, we observed a new pathway-MSTRG.598.1/Lilrb2 pathway with the HYJJ treatment; however, it needs further studies. In conclusion, this study provided evidence that HYJJ may be a suitable medicine for treating CHF. Moreover, several pivotal miRNAs may serve important roles in these processes by regulating some key miRNAs or pathways in CHF.
回阳救急（HYJJ）汤已被应用于治疗慢性心力衰竭（CHF）的中药方剂。然而，其综合分子机制目前尚不清楚。我们的研究旨在探讨 HYJJ 对阿霉素（DOX）诱导的大鼠 CHF 的可能作用及 lncRNA-miRNA 调控网络。我们的研究表明，HYJJ 可恢复心脏功能并减轻 DOX 诱导的 CHF 心肌损伤。此外，HYJJ 对 CHF 大鼠心肌细胞凋亡有抑制作用。此外，RNA 测序和生物信息学分析表明，在总共 548 个显著上调和下调的差异表达（DE）长非编码 RNA（lncRNA）中，鉴定出 511 个上调和下调的 DE-miRNAs。DE-lncRNA 富集途径和 DE-miRNA 富集途径之间的共同途径是库欣综合征和心肌细胞肾上腺素能信号转导。最后，我们观察到 HYJJ 治疗后出现了一条新的途径-MSTRG.598.1/Lilrb2 途径；然而，这需要进一步研究。总之，本研究为 HYJJ 可能是治疗 CHF 的一种合适药物提供了证据。此外，一些关键的 miRNAs 可能通过调节 CHF 中的某些关键 miRNAs 或途径，在这些过程中发挥重要作用。
