Bing Tao, Zhang Nan, Shangguan Dihua
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Adv Biosyst. 2019 Dec;3(12):e1900193. doi: 10.1002/adbi.201900193. Epub 2019 Nov 12.
Cell-SELEX can not only generate aptamers for specific cell isolation/detection, diagnosis, and therapy, but also lead to the discovery of biomarkers and unexpected molecular events. However, most cell-SELEX research is concentrated on aptamer generation and applications. In this progress report, recent research progress with cell-SELEX in terms of the discovery of biomarkers and unexpected molecular events is highlighted. In particular, the key technical challenges for cell-SELEX-based biomarker discovery, namely, the methods for identification and validation of target proteins of aptamers, are discussed in detail. Finally, the prospects of the applications of cell-SELEX in this field now and in the near future are described. It is expected that this report will attract attention to the benefit of cell-SELEX and provide a practical reference for biomedical researchers.
细胞指数富集的配体系统进化技术（Cell-SELEX）不仅可以生成用于特定细胞分离/检测、诊断和治疗的适配体，还能促使生物标志物和意外分子事件的发现。然而，大多数细胞指数富集的配体系统进化技术研究都集中在适配体的生成和应用上。在本进展报告中，重点介绍了细胞指数富集的配体系统进化技术在生物标志物和意外分子事件发现方面的最新研究进展。特别是，详细讨论了基于细胞指数富集的配体系统进化技术进行生物标志物发现的关键技术挑战，即适配体靶蛋白的鉴定和验证方法。最后，描述了细胞指数富集的配体系统进化技术在该领域当前及不久将来的应用前景。预计本报告将引起人们对细胞指数富集的配体系统进化技术优势的关注，并为生物医学研究人员提供实用参考。
