Lack of Known Target-Site Mutations in Field Populations of in China from 2019 to 2021.

The Asian corn borer, (Guenée) (Lepidoptera; Pyralidae), is one of the most destructive insect pests of corn, for which chemical insecticides have been the primary method of control, especially during outbreaks. Little information is currently available on the status of insecticide resistance and associated mechanisms in field populations. Invasions and outbreaks of in China in recent years have increased chemical application in corn fields, which adds to the selection pressure on . This study was conducted to estimate the risk of insecticide resistance by investigating the frequency of insecticide resistant alleles associated with target site insensitivity in field populations of . Using the individual-PCR genotype sequencing analysis, none of the six target-site insecticide resistant mutations were detected in field populations collected from 2019 to 2021 in China. These investigated insecticide resistance alleles are common in resistant Lepidoptra pests and are responsible for resistance to pyrethroids, organophosphorus, carbamates, diamide, and Cry1Ab. Our results support the low insecticide resistance status in field populations and betokens the unlikely development of high resistance mediated by the common target-site resistance alleles. Additionally, the findings would serve as references for further efforts toward the sustainable management of .