Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe Shim-machi, Suita, Osaka 5645-8565, Japan.
Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
Biomacromolecules. 2020 Aug 10;21(8):3092-3101. doi: 10.1021/acs.biomac.0c00457. Epub 2020 Jul 22.
Early-stage hemocompatibility is indispensable for manufacturing tissue-engineered vascular grafts used in regenerative medicine. In this study, we report the blood response and patency of small-diameter synthetic vascular grafts modified with the Arg-Glu-Asp-Val (REDV) peptide. Vascular grafts were prepared by casting REDV-conjugated poly(depsipeptide--caprolactone) on a stainless-steel mandril (diameter: 1.8 mm). After implanting the grafts into the abdominal aorta of rats for 24 h, all three control grafts without the peptide and three out of the four REDV (control sequence) peptide-modified grafts showed occlusion. The luminal surfaces of these grafts were covered with thick thrombi. In contrast, all the grafts containing the REDV peptide were patent, and their luminal surfaces were covered with a thin layer of fibrin. These results indicated that the REDV peptide on the luminal surface effectively reduced early-stage fibrin clot deposition and formed the pseudo-endothelium layer in a peptide sequence-specific manner, resulting in graft patency.
早期血液相容性对于制造用于再生医学的组织工程血管移植物是必不可少的。在这项研究中，我们报告了用 Arg-Glu-Asp-Val（REDV）肽修饰的小直径合成血管移植物的血液反应和通畅率。通过将 REDV 缀合的聚（去肽 - 己内酯）涂覆在不锈钢心轴（直径：1.8 毫米）上制备血管移植物。将移植物植入大鼠腹主动脉 24 小时后，没有肽的三个对照移植物和四个 REDV（对照序列）肽修饰移植物中的三个都发生了闭塞。这些移植物的管腔表面被厚厚的血栓覆盖。相比之下，所有含有 REDV 肽的移植物都是通畅的，其管腔表面覆盖着一层薄薄的纤维蛋白。这些结果表明，管腔表面的 REDV 肽可有效减少早期纤维蛋白凝块的沉积，并以肽序列特异性的方式形成假内皮层，从而使移植物保持通畅。
