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一种新型 GATA2 蛋白报告小鼠揭示了造血祖细胞类型。

A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types.

机构信息

Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland.

Biotechnology Center, Technische Universität Dresden, Tatzberg 47-51, 01307 Dresden, Germany.

出版信息

Stem Cell Reports. 2020 Aug 11;15(2):326-339. doi: 10.1016/j.stemcr.2020.06.008. Epub 2020 Jul 9.

DOI:10.1016/j.stemcr.2020.06.008
PMID:32649900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7419669/
Abstract

The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endogenous Gata2 genomic locus, with correct expression and localization of GATA2VENUS in different organs. GATA2VENUS expression is heterogeneous in different hematopoietic stem and progenitor cell populations (HSPCs), identifies functionally distinct subsets, and suggests a novel monocyte and mast cell lineage bifurcation point. GATA2 levels further correlate with proliferation and lineage outcome of hematopoietic progenitors. The GATA2VENUS mouse line improves the identification of specific live cell types during embryonic and adult development and will be crucial for analyzing GATA2 protein dynamics in TF networks.

摘要

转录因子 (TF) GATA2 在中枢神经系统、泌尿生殖系统、呼吸系统和生殖系统的器官发育和细胞命运控制中，以及在原始和定型造血中发挥关键作用。在这里，我们从内源性 Gata2 基因组座产生了一种表达 GATA2VENUS 融合蛋白的基因敲入蛋白报告小鼠系，GATA2VENUS 在不同器官中的表达和定位正确。GATA2VENUS 在不同的造血干/祖细胞群体 (HSPCs) 中的表达具有异质性，鉴定出功能不同的亚群，并提示单核细胞和肥大细胞谱系分叉点。GATA2 水平进一步与造血祖细胞的增殖和谱系结果相关。GATA2VENUS 小鼠系可改善胚胎和成年期特定活细胞类型的鉴定，对于分析 TF 网络中的 GATA2 蛋白动力学至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/7a27356548ac/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/aae4c553b728/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/369675c06c2b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/7c80aa995926/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/d86667b437ae/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/c8d98c2ece9b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/73fea0bf12a2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/7a27356548ac/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/aae4c553b728/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/369675c06c2b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/7c80aa995926/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/d86667b437ae/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/c8d98c2ece9b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/73fea0bf12a2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e982/7419669/7a27356548ac/gr7.jpg

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