Domingues William B, Blodorn Eduardo B, Martins Amanda S W, Dellagostin Eduardo N, Komninou Eliza R, Hurtado Joaquin I, Corcini Carine D, Varela Junior Antonio S, Pinto Luciano S, Kremer Frederico S, Collares Tiago, Pinhal Danillo, Greif Gonzalo, Robello Carlos, Schneider Augusto, Guo Su, Campos Vinicius F
Laboratório de Genômica Estrutural, Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Laboratório de Reprodução Animal, Programa de Pós-Graduação em Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Theriogenology. 2020 Oct 15;156:11-19. doi: 10.1016/j.theriogenology.2020.06.025. Epub 2020 Jun 29.
MicroRNAs have been hypothesized to be involved in the regulation of male fertility potential. The primary aim of our study was to demonstrate the effects of transfection with dendrimer nanostructure on the parameters of bovine sperm quality and to investigate whether the microRNA profile could be disturbed after cationic dendrimer-mediated exogenous DNA transfection of bovine spermatozoa. The binding of exogenous DNA was significantly increased when dendrimer-based transfection was implemented. However, cationic dendrimer transfection induced detrimental changes in the kinetics and sperm quality parameters, such as membrane integrity, acrosome reaction, and mitochondrial membrane potential, when compared to the control group. Sperm microRNA sequencing revealed 218 known and 106 novel microRNAs in the sperm samples, among which nine were dysregulated after transfection (one was upregulated and eight were downregulated), in comparison to the non-transfected sperm. All the dysregulated microRNAs were related to sperm quality and embryonic development. These results suggest that the transfection process using the dendrimer nanostructure has an impact on the quality and microRNA profile of bovine sperm.
已有假说认为，微小RNA参与雄性生育潜能的调控。我们研究的主要目的是证明用树枝状纳米结构转染对牛精子质量参数的影响，并研究在阳离子树枝状聚合物介导的牛精子外源性DNA转染后，微小RNA谱是否会受到干扰。当实施基于树枝状聚合物的转染时，外源性DNA的结合显著增加。然而，与对照组相比，阳离子树枝状聚合物转染在动力学和精子质量参数方面引起了有害变化，如膜完整性、顶体反应和线粒体膜电位。精子微小RNA测序在精子样本中发现了218种已知微小RNA和106种新的微小RNA，与未转染的精子相比，其中9种在转染后表达失调（1种上调，8种下调）。所有失调的微小RNA都与精子质量和胚胎发育有关。这些结果表明，使用树枝状纳米结构的转染过程对牛精子的质量和微小RNA谱有影响。
