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精原细胞瘤和非精原细胞瘤性睾丸生殖细胞肿瘤患者精子的蛋白质组特征明显不同。

Distinct Proteomic Profile of Spermatozoa from Men with Seminomatous and Non-Seminomatous Testicular Germ Cell Tumors.

机构信息

American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.

Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar and Unit for Multidisciplinary Research in Biomedicine (UMIB), University of Porto, 4050-313 Porto, Portugal.

出版信息

Int J Mol Sci. 2020 Jul 8;21(14):4817. doi: 10.3390/ijms21144817.

DOI:10.3390/ijms21144817
PMID:32650378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7404221/
Abstract

Testicular germ cell tumors (TGCTs) are predominant in young males (15-44 years). Seminomatous and non-seminomatous TGCTs account for about 98% of all TGCTs cases. In this study, we aimed to compare the sperm proteome of patients with seminomatous and non-seminomatous TGCTs to identify possible protein biomarkers that could help distinguish between them in a non-invasive manner. We analyzed semen samples from patients with seminomatous or non-seminomatous TGCTs ( = 15/group) that were cryopreserved before the start of cancer treatment. Quantitative proteomic analysis was conducted on pooled samples ( = 3/group) and a total of 258 differentially expressed proteins (DEPs) were identified. The overexpression of acrosin precursor (ACR) and chaperonin containing TCP1 subunit 6B (CCT6B) as well as the underexpression of S100 calcium-binding protein A9 (S100A9) in the spermatozoa of patients with non-seminomatous TGCTs were validated by western blotting conducted on individual samples ( = 6 for seminomatous group and = 6 for non-seminomatous group). Our overall results suggest an association between the higher and faster invasiveness of non-seminomatous TGCTs and the altered protein expressions, providing important information for future studies.

摘要

睾丸生殖细胞肿瘤 (TGCTs) 主要发生在年轻男性（15-44 岁）中。精原细胞瘤和非精原细胞瘤 TGCTs 约占所有 TGCTs 病例的 98%。在这项研究中，我们旨在比较精原细胞瘤和非精原细胞瘤 TGCT 患者的精子蛋白质组，以确定可能的蛋白质生物标志物，以便以非侵入性的方式帮助区分它们。我们分析了冷冻保存于癌症治疗开始前的精原细胞瘤或非精原细胞瘤 TGCT 患者（每组 15 例）的精液样本。对合并样本（每组 3 例）进行定量蛋白质组学分析，共鉴定出 258 个差异表达蛋白 (DEPs)。非精原细胞瘤 TGCT 患者精子中顶体酶前体 (ACR) 和含 TCP1 亚基的热休克蛋白 6B (CCT6B) 的过表达以及 S100 钙结合蛋白 A9 (S100A9) 的低表达通过对个体样本进行 Western blot 验证（精原细胞瘤组为 6 例，非精原细胞瘤组为 6 例）。我们的总体结果表明，非精原细胞瘤 TGCT 的更高侵袭性和更快侵袭性与蛋白质表达的改变之间存在关联，为未来的研究提供了重要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3d6/7404221/4c2576fbe099/ijms-21-04817-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3d6/7404221/76d4a7d1be42/ijms-21-04817-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3d6/7404221/4c2576fbe099/ijms-21-04817-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3d6/7404221/76d4a7d1be42/ijms-21-04817-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3d6/7404221/4c2576fbe099/ijms-21-04817-g002.jpg

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