Department of Hepatobiliary Surgery, The Second People's Hospital of Wuhu, No. 259, Jiuhua Middle Road, Wuhu, 241001, Anhui, China.
Biotechnol Lett. 2020 Nov;42(11):2447-2452. doi: 10.1007/s10529-020-02954-6. Epub 2020 Jul 10.
To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway.
Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method.
Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR.
In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.
探讨 Ag-1031 对胃癌 AGS 细胞凋亡及 PI3K/AKT/mTOR 信号通路的影响。
用不同浓度的 Ag-1031 处理胃癌细胞。用 MTT 法检测细胞活力。用 Hoechst 33342 染色和 Annexin V-FITC/PI 双染法分析细胞凋亡。用 Western blot 法检测 p-PI3K、p-AKT、p-mTOR、PI3K、AKT 和 mTOR 的蛋白表达水平。
不同浓度的 Ag-1031 显著抑制 AGS 细胞的增殖。此外，Ag-1031 诱导胃癌细胞凋亡。此外，Ag-1031 显著且呈剂量依赖性抑制 p-PI3K、p-AKT 和 p-mTOR 的蛋白表达，但对 PI3K、AKT 和 mTOR 的蛋白表达没有影响。
本研究首次发现，Ag-1031 通过抑制 PI3K/AKT/mTOR 信号通路的激活诱导胃癌 AGS 细胞凋亡发挥抗肿瘤作用。
