School of Basic Medical Sciences, Wannan Medical College, Wuhu, 241002, China.
College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, China.
World J Microbiol Biotechnol. 2023 Nov 4;40(1):1. doi: 10.1007/s11274-023-03792-0.
The selection of effective antibiotics is becoming increasingly limited due to the emergence of bacterial resistance. Designing and developing nanoscale antibacterials is a strategy for effectively addressing the antibiotic crisis. In this work, AgNPs@AMP nanoparticles were synthesized to take advantage of the synergistic antibacterial activity of the (LLRR) antimicrobial peptide (AMP) and silver nanoparticles (AgNPs). Based on morphological structure characterization and biocompatibility analysis, the inhibitory properties of AgNPs@AMP on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were evaluated. The results demonstrated that AMP and AgNPs were physically bound to form AgNPs@AMP nanoparticles, which had better solution stability, improved nanomaterial properties, and overcame the hemolytic activity of AMP and the cytotoxicity of AgNPs. The inhibitory activity of AgNPs@AMP against E. coli and S. aureus was significantly higher than that of AMP and AgNPs. It was capable of disrupting the morphology and internal structure of cells, damaging the cell membrane, and inhibiting the activity of enzymes related to the material-energy metabolism of the tricarboxylic acid cycle. Compared to AMP and AgNPs, AgNPs@AMP were found to effectively inhibit the infection of mouse wounds and promote their healing. Therefore, AMP-modified AgNPs can enhance their biocompatibility and antibacterial activity, and they can be further developed as a potential antimicrobial agent.
由于细菌耐药性的出现，有效抗生素的选择变得越来越有限。设计和开发纳米级抗菌剂是有效应对抗生素危机的一种策略。在这项工作中，合成了 AgNPs@AMP 纳米粒子，以利用抗菌肽 (AMP) 和银纳米粒子 (AgNPs) 的协同抗菌活性。基于形态结构表征和生物相容性分析，评估了 AgNPs@AMP 对大肠杆菌 (E. coli) 和金黄色葡萄球菌 (S. aureus) 的抑制特性。结果表明，AMP 和 AgNPs 通过物理结合形成 AgNPs@AMP 纳米粒子，具有更好的溶液稳定性、改善的纳米材料性质，并克服了 AMP 的溶血活性和 AgNPs 的细胞毒性。AgNPs@AMP 对 E. coli 和 S. aureus 的抑制活性明显高于 AMP 和 AgNPs。它能够破坏细胞的形态和内部结构，损伤细胞膜，并抑制与三羧酸循环物质能量代谢相关的酶的活性。与 AMP 和 AgNPs 相比，AgNPs@AMP 被发现能有效抑制小鼠伤口感染并促进其愈合。因此，AMP 修饰的 AgNPs 可以提高其生物相容性和抗菌活性，并可进一步开发为一种有潜力的抗菌剂。
