Laboratory of Physiology of Cardiovascular and Lymphatic Systems, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.
Bull Exp Biol Med. 2020 Jun;169(2):192-196. doi: 10.1007/s10517-020-04848-z. Epub 2020 Jul 10.
We studied the effects and mechanisms of action of NaHS, an HS donor, on isolated phenylephrine-precontracted bovine mesenteric lymph nodes. NaHS induced concentration-dependent relaxation of lymph nodes. Removal of the endothelium reduced, but did not abolish the relaxing effect of NaHS. The relaxing effect was reduced by NO synthase inhibitor L-NAME, soluble guanylate cyclase inhibitor ODQ, ATP-sensitive K channel blocker glibenclamide, and a combination charybdotoxin+apamin (blockers of small- and intermediate-conductance Ca-activated K channels). Thus, the relaxing effect of HS on lymph nodes is mediated by several parallel mechanisms. HS induces relaxation of LN and modulates the rate of lymph transport, thereby affecting the development of immune processes in the body.
我们研究了 HS 供体 NaHS 对分离的苯肾上腺素预收缩牛肠系膜淋巴结的作用和作用机制。NaHS 诱导淋巴结浓度依赖性松弛。内皮细胞去除减少了，但并没有消除 NaHS 的松弛作用。NO 合酶抑制剂 L-NAME、可溶性鸟苷酸环化酶抑制剂 ODQ、ATP 敏感性 K 通道阻滞剂格列本脲以及混合 charybdotoxin+apamin（小电导和中等电导 Ca 激活 K 通道阻滞剂）减少了松弛作用。因此，HS 对淋巴结的松弛作用是由几种平行机制介导的。HS 诱导 LN 松弛并调节淋巴转运率，从而影响体内免疫过程的发展。
