Sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan from seafood Amphioctopus neglectus attenuates angiotensin-II prompted cardiac hypertrophy.

Angiotensin converting enzyme (ACE) is a multifunctional enzyme involved in translation of angiotensin-I (Ang) to vasoconstrictor angiotensin-II (Ang). A sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan characterized as poly-[(2-methoxy-β-arabinopyranosyl)-(1 → 3)-(β-glucurono)-(1 → 4)-(2-acetamido-2-deoxy-3,6-di-O-sulfonato-β-glucopyranose)] was purified and reported first time from the edible portion of Amphioctopus neglectus and evaluated for various pharmacological properties. The polysaccharide exhibited potential ACE attenuation property (IC 0.11 mg mL), whereas molecular docking simulations displayed its efficient binding at the ACE active site with lesser inhibitory constant (K) of 17.36 nM and binding energy (-10.59 kcal mol). The in-vitro analysis showed that the studied polysacharide attenuated Ang prompted cardiac hypertrophy at 50 μg mL in the cardiomyoblast cells, whereas 48% reduction in cellular surface area with extended viability could be correlated with anti-hypertrophic properties of the studied polysaccharide. The sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan purified from A. neglectus could function as a prospective functional lead against the pathophysiological conditions leading to hypertension.