由单克隆抗Thy 1.1抗体与核糖体失活蛋白苔藓抑素和苦瓜素组成的免疫毒素的选择性细胞毒性活性。
Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-Thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin.
作者信息
Stirpe F, Wawrzynczak E J, Brown A N, Knyba R E, Watson G J, Barbieri L, Thorpe P E
机构信息
Dipartimento di Patologia Sperimentale, Universita degli Studi di Bologna, Italy.
出版信息
Br J Cancer. 1988 Nov;58(5):558-61. doi: 10.1038/bjc.1988.258.
The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKR-A cells by 50% at a concentration of 1-4 x 10(-11) M as compared with 1 x 10(-9) M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10(-8) M. Further, bryodin and momordin immunotoxins made from an antibody (R10) of irrelevant specificity were without effect on AKR-A cells.
来自白泻根的核糖体失活蛋白 bryodin 和来自苦瓜的天花粉蛋白通过二硫键与单克隆抗 Thy 1.1 抗体（OX7）偶联。两种免疫毒素在体外对表达 Thy 1.1 的小鼠淋巴瘤细胞系 AKR-A 均具有特异性细胞毒性。OX7-bryodin 免疫毒素毒性更强，在浓度为 1 - 4×10⁻¹¹ M 时可使 AKR-A 细胞中的蛋白质合成减少 50%，而 OX7-天花粉蛋白免疫毒素则需 1×10⁻⁹ M。在浓度高达 3×10⁻⁸ M 时，两种免疫毒素对缺乏 Thy 1.1 抗原的小鼠淋巴瘤 EL4 细胞均无毒性。此外，由不相关特异性抗体（R10）制备的 bryodin 和天花粉蛋白免疫毒素对 AKR-A 细胞没有影响。
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