核糖体失活蛋白（相思子毒素、苦瓜抑制剂和天花粉蛋白）的特性。
Properties of the ribosome-inactivating proteins gelonin, Momordica charantia inhibitor, and dianthins.
作者信息
Falasca A, Gasperi-Campani A, Abbondanza A, Barbieri L, Stirpe F
出版信息
Biochem J. 1982 Dec 1;207(3):505-9. doi: 10.1042/bj2070505.
The amino acid and sugar compositions of four ribosome-inactivating proteins (gelonin, Momordica charantia inhibitor, dianthin 30 and dianthin 32) were determined. The proteins are all basic glycoproteins (pI greater than 8) containing mannose (more abundant in gelonin), glucose, xylose, fucose (absent from gelonin) and glucosamine. The ribosome-inactivating properties of the proteins examined are not modified by pretreatment with N-ethylmaleimide. Precipitating and inactivating antibodies can be raised against ribosome-inactivating proteins; a weak cross-reaction was observed only between dianthin 30 and dianthin 32.
测定了四种核糖体失活蛋白（相思豆毒蛋白、苦瓜抑制剂、石竹素30和石竹素32）的氨基酸和糖组成。这些蛋白均为碱性糖蛋白（pI大于8），含有甘露糖（在相思豆毒蛋白中含量更高）、葡萄糖、木糖、岩藻糖（相思豆毒蛋白中不存在）和氨基葡萄糖。所检测蛋白的核糖体失活特性不会因用N-乙基马来酰亚胺预处理而改变。可以产生针对核糖体失活蛋白的沉淀性和失活性抗体；仅在石竹素30和石竹素32之间观察到微弱的交叉反应。
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