College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China.
College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China.
Sci Total Environ. 2020 Nov 15;743:140692. doi: 10.1016/j.scitotenv.2020.140692. Epub 2020 Jul 3.
Anthrax spores pose a serious threat to human well-being and life, so it is highly desirable to develop a rapid, sensitive, and selective quantitative assay of calcium pyridine dicarboxylate (CaDPA) as a biomarker of anthrax spores. Herein, carbon dots (CDs) chelated Eu@metal-organic framework (Eu-MOFs) as dual-emissive ratiometric fluorescent (RF) probe was successfully fabricated by a simple one-pot in situ selective self-assembly synthetic strategy. The developed RF probe has an effective self-calibration function, which performs a highly sensitive and selective recognition of CaDPA in water and human serum sample. The blue-emitting CDs was employed as an effective fluorescence reference, while the Eu-MOFs exhibited enhanced red fluorescence signal through the coordination interaction with CaDPA chromophore. The sensing mechanism is attributed to that CaDPA can sensitize Eu intrinsic luminescence due to the energy transfer from CaDPA to Eu. What's more interesting is that with the continuous drop of CaDPA, the emission color of CDs@Eu-MOF changes from blue to red. The results revealed that CDs@Eu-MOFs RF probe can detect CaDPA effectively in the range of 8-170 μg/L with good linear relationship, and exhibited a remarkable selectivity for CaDPA. More interestingly, a paper-based probe has also been devised for on-site detection of CaDPA. In addition, CaDPA is used as input signal to construct an IHIBITION logic gate device which performs the "off-on" mode. The constructed CDs@Eu-MOF probe can achieve exceptionally rapid, highly sensitive and selective detection of CaDPA, which can further expand the application prospects in environmental and biological analysis.
炭疽芽孢对人类健康和生命构成严重威胁，因此，开发一种快速、灵敏、选择性定量测定吡啶二羧酸钙（CaDPA）作为炭疽芽孢生物标志物的方法是非常可取的。在此，通过简单的一锅原位选择性自组装合成策略，成功制备了碳点（CDs）螯合 Eu@金属有机骨架（Eu-MOFs）的双发射比率荧光（RF）探针。所开发的 RF 探针具有有效的自校准功能，可在水和人血清样品中对 CaDPA 进行高灵敏度和选择性识别。蓝色发射的 CDs 用作有效的荧光参考，而 Eu-MOFs 通过与 CaDPA 发色团的配位相互作用表现出增强的红色荧光信号。传感机制归因于 CaDPA 可以由于能量从 CaDPA 转移到 Eu 而敏化 Eu 的本征发光。更有趣的是，随着 CaDPA 的不断下降，CDs@Eu-MOF 的发射颜色从蓝色变为红色。结果表明，CDs@Eu-MOFs RF 探针可以在 8-170μg/L 的范围内有效检测 CaDPA，具有良好的线性关系，并对 CaDPA 表现出显著的选择性。更有趣的是，还设计了一种用于现场检测 CaDPA 的纸基探针。此外，CaDPA 被用作输入信号来构建 IHIBITION 逻辑门器件，该器件执行“关-开”模式。构建的 CDs@Eu-MOF 探针可以实现对 CaDPA 的快速、高灵敏和选择性检测，这可以进一步扩展其在环境和生物分析中的应用前景。
