Cao Chunhao, Sun Wei, Yang Ling, Yang Qiliang
Yunnan Key Laboratory of Efficient Utilization and Intelligent Control of Agricultural Water Resources, Kunming University of Science and Technology, Kunming 650500, China.
Seasonal Arid Region, Water-Soil-Crop System Observation and Research Station of Yunnan Province, Kunming University of Science and Technology, Kunming 650500, China.
Plants (Basel). 2025 Jul 26;14(15):2316. doi: 10.3390/plants14152316.
The leaf diseases of ( (Burk) F. H. Chen) are mainly spread by spores. To enable rapid and sensitive detection of spores for early warning of disease spread, we developed a carbon dot-based fluorescent probe encapsulated by MIL-101 using wax apple as a green carbon source (WA-CDs@MIL-101). The WA-CDs@MIL-101 was thoroughly characterized, and the detection conditions were optimized. The interaction mechanism between WA-CDs@MIL-101 and spores was investigated. The fluorescence of WA-CDs@MIL-101 was recovered due to electrostatic adsorption between spores and WA-CDs@MIL-101. Under the optimized detection conditions, the probe exhibited excellent sensing performance, showing a strong linear relationship ( = 0.9978) between spore concentration (0.0025-5.0 mg/L) and fluorescence recovery ratio, with a detection limit of 5.15 μg/L. The WA-CDs@MIL-101 was successfully applied to detect spores on leaves, achieving satisfactory recoveries (94-102%) with relative standard deviations of 1.3-3.4%. The WA-CDs@MIL-101 shows great promise for detecting spores on leaves.
莲雾（（Burk）F. H. Chen）的叶部病害主要通过孢子传播。为了能够快速、灵敏地检测孢子以对病害传播进行早期预警，我们以莲雾为绿色碳源开发了一种由MIL-101封装的基于碳点的荧光探针（WA-CDs@MIL-101）。对WA-CDs@MIL-101进行了全面表征，并优化了检测条件。研究了WA-CDs@MIL-101与孢子之间的相互作用机制。由于孢子与WA-CDs@MIL-101之间的静电吸附作用，WA-CDs@MIL-101的荧光得以恢复。在优化的检测条件下，该探针表现出优异的传感性能，孢子浓度（0.0025 - 5.0 mg/L）与荧光恢复率之间呈现出很强的线性关系（ = 0.9978），检测限为5.15 μg/L。WA-CDs@MIL-101成功应用于检测莲雾叶片上的孢子，回收率令人满意（94 - 102%），相对标准偏差为1.3 - 3.4%。WA-CDs@MIL-101在检测莲雾叶片上的孢子方面具有很大的应用前景。
