海兔糖胺聚糖对 ADP 诱导的大鼠血小板钙离子和膜糖蛋白表达的影响。
Effects of Glycosaminoglycan from Urechis unicinctus on ADP-Induced Platelet Calcium and Membrane Glycoprotein Expressions in Rats.
机构信息
Tianjin Key Laboratory of Marine Resource and Chemistry, Tianjin Marine Environmental Protection and Restoration Technology Engineering Center, College of Marine and Environment, Tianjin University of Science and Technology, Tianjin, China,
Tianjin Key Laboratory of Marine Resource and Chemistry, Tianjin Marine Environmental Protection and Restoration Technology Engineering Center, College of Marine and Environment, Tianjin University of Science and Technology, Tianjin, China.
出版信息
Acta Haematol. 2021;144(1):44-47. doi: 10.1159/000505769. Epub 2020 Jul 10.
BACKGROUND
Previous studies had shown that glycosaminoglycan (GAG) from Urechis unicinctus exerts an obvious antiplatelet aggregation effect.
OBJECTIVE
This study aims to investigate the effects of GAG from Urechis unicinctus on ADP-induced platelet calcium and membrane glycoprotein expressions in rats.
METHODS
Fura-2/AM fluorescence probe was used to measure intracytosolic free-calcium concentration ([Ca2+]i) of platelets and calculate platelet calcium influx and release concentrations. Flow cytometry was used to detect the expressions of platelet membrane glycoproteins GPIIb/IIIa (PAC-1) and P-selectin (CD62P) in rats.
RESULTS
The results showed that the GAG from U. unicinctus significantly inhibited the release of platelet calcium (p < 0.01) and the expressions of platelet GPII b/IIIa and P-selectin (p < 0.01) induced by ADP in rats but had no significant effect on the influx of platelet calcium (p > 0.01).
CONCLUSIONS
This study indicated that GAG may inhibit platelet activation and aggregation by reducing the release of Ca2+ and ADP-induced expression of platelet membrane glycoprotein in rats.
背景
先前的研究表明，来自单环刺螠的糖胺聚糖（GAG）具有明显的抗血小板聚集作用。
目的
本研究旨在探讨单环刺螠 GAG 对 ADP 诱导的大鼠血小板钙和膜糖蛋白表达的影响。
方法
使用 Fura-2/AM 荧光探针测量血小板细胞内游离钙浓度([Ca2+]i)，并计算血小板钙内流和释放浓度。流式细胞术检测大鼠血小板膜糖蛋白 GPIIb/IIIa（PAC-1）和 P-选择素（CD62P）的表达。
结果
结果表明，单环刺螠 GAG 显著抑制 ADP 诱导的大鼠血小板钙释放（p<0.01）和血小板 GPIIb/IIIa 和 P-选择素的表达（p<0.01），但对血小板钙内流无明显影响（p>0.01）。
结论
本研究表明，GAG 可能通过减少 Ca2+ 的释放和 ADP 诱导的血小板膜糖蛋白表达来抑制血小板的激活和聚集。
Zotero 插件