Mun Jongwon, Pack Seung Pil, Lee Hyeongjin
Department of Biotechnology and Bioinformatics, Korea University Sejong 30019 Republic of Korea
RSC Adv. 2025 Aug 29;15(38):31060-31075. doi: 10.1039/d5ra04406e.
Small-diameter vascular grafts (SDVGs; ≤6 mm inner diameter) often fail due to thrombosis, poor endothelialization, and low patency. To overcome these limitations, we developed electrospun composite scaffolds incorporating decellularized ECM (UdECM), a marine invertebrate source rich in collagen, glycosaminoglycans, and elastin. UdECM was blended with polycaprolactone (PCL) at 1, 5, and 10 wt% and electrospun into fibrous matrices. We characterized scaffold hydrophilicity (water contact angle, water uptake), mechanical properties, and cell behaviors (, viability, proliferation, tube formation) using EA.hy926 endothelial cells, and performed blood clotting assays. UdECM-containing scaffolds exhibited improved hydrophilicity and mechanical strength compared to pure PCL, with the 10 wt% UdECM scaffold demonstrating the highest stiffness while retaining suitable elongation. Endothelial cells grown on UdECM-enhanced scaffolds showed increased viability and tube formation, indicative of a pro-angiogenic environment. Anticoagulant tests revealed reduced blood cell adhesion with higher UdECM content. These findings underscore the potential of marine-derived ECM to enhance the functionality of synthetic vascular grafts by promoting both endothelialization and thromboresistance.
小口径血管移植物（内径≤6毫米）常因血栓形成、内皮化不良和通畅率低而失效。为克服这些局限性，我们开发了一种电纺复合支架，其包含脱细胞细胞外基质（UdECM），这是一种富含胶原蛋白、糖胺聚糖和弹性蛋白的海洋无脊椎动物来源。将UdECM与聚己内酯（PCL）按1%、5%和10%的重量比混合，并静电纺丝成纤维基质。我们使用EA.hy926内皮细胞对支架的亲水性（水接触角、吸水率）、力学性能和细胞行为（活力、增殖、成管）进行了表征，并进行了凝血试验。与纯PCL相比，含UdECM的支架表现出更好的亲水性和机械强度，其中10%重量比的UdECM支架在保持适当伸长率的同时显示出最高的硬度。在UdECM增强支架上生长的内皮细胞显示出活力增加和成管能力增强，这表明存在促血管生成环境。抗凝测试表明，随着UdECM含量的增加，血细胞粘附减少。这些发现强调了海洋来源的细胞外基质通过促进内皮化和抗血栓形成来增强合成血管移植物功能的潜力。
