Bauer A, Rutenfranz I, Kirchner H
Institute of Virus Research, German Cancer Research Center, Heidelberg, FRG.
Eur J Immunol. 1988 Dec;18(12):2109-12. doi: 10.1002/eji.1830181239.
Mycoplasma arthritidis produces in culture a polyclonal mitogen which is active for murine and human T lymphocytes in the presence of accessory cells (AC). We studied the requirements for processing and presentation by AC of Mycoplasma arthritidis supernatant (MAS) mitogen to human T cells. As inhibitors of AC processing, several agents were used which inhibit lysosomal function: the weak bases chloroquine and NH4Cl, the cationic ionophore monensin and the competitive protease inhibitor leupeptin. When these agents were used to inhibit processing by presenting cells and washed out before T cells were added to culture, they inhibited lymphocyte activation and, therefore, we assume that they interfered with the presentation of the mitogen. Thus, if MAS requires a processing step, it appears to involve lysosomal proteolysis which can be blocked in vitro.
关节炎支原体在培养过程中产生一种多克隆有丝分裂原，在辅助细胞（AC）存在的情况下，该有丝分裂原对小鼠和人类T淋巴细胞具有活性。我们研究了AC将关节炎支原体上清液（MAS）有丝分裂原加工处理并呈递给人类T细胞的条件。作为AC加工处理的抑制剂，我们使用了几种抑制溶酶体功能的试剂：弱碱氯喹和氯化铵、阳离子离子载体莫能菌素以及竞争性蛋白酶抑制剂亮抑酶肽。当使用这些试剂抑制递呈细胞的加工处理，并在将T细胞加入培养物之前将其洗脱时，它们抑制了淋巴细胞的活化，因此，我们认为它们干扰了有丝分裂原的呈递。因此，如果MAS需要一个加工步骤，那么这个步骤似乎涉及可在体外被阻断的溶酶体蛋白水解作用。
