Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Arch Physiol Biochem. 2023 Feb;129(1):10-15. doi: 10.1080/13813455.2020.1791187. Epub 2020 Jul 11.
Regarding the central role of insulin resistance in NAFLD, we explored whether insulin-like growth factor 1 () and insulin-like growth factor-binding protein 3 () gene variants were associated with NAFLD susceptibility.
(rs6214) and (rs3110697) gene variants were genotyped in 154 cases with biopsy-proven NAFLD and 156 controls using PCR-RFLP method.
The rs6214 "AA + AG" genotype compared with the "GG" genotype appeared to be a marker of decreased NAFLD susceptibility ( = .006; OR = 0.47, 95%CI = 0.28-0.80). Furthermore, the rs6214 "A" allele was underrepresented in the cases than controls ( = .024; OR = 0.61, 95%CI = 0.40-0.94). However, we observed no significant difference in genotype or allele frequencies between the cases and controls for gene.
To our knowledge, these findings suggest, for the first time, that the rs6214 "A" allele and "AA + AG" genotype have protective effects for NAFLD susceptibility. Nonetheless, further studies are needed to validate our findings.
鉴于胰岛素抵抗在非酒精性脂肪性肝病（NAFLD）中的核心作用，我们探讨了胰岛素样生长因子 1（IGF-1）和胰岛素样生长因子结合蛋白 3（IGFBP-3）基因变异是否与 NAFLD 的易感性相关。
采用 PCR-RFLP 方法，对 154 例经肝活检证实的 NAFLD 患者和 156 例对照者的 （rs6214）和 （rs3110697）基因变异进行基因分型。
与“GG”基因型相比，rs6214“AA+AG”基因型似乎是降低 NAFLD 易感性的标志物（=0.006；OR=0.47，95%CI=0.28-0.80）。此外，rs6214“A”等位基因在病例组中的频率低于对照组（=0.024；OR=0.61，95%CI=0.40-0.94）。然而，我们未观察到病例组和对照组之间 rs6214 基因的基因型或等位基因频率存在显著差异。
据我们所知，这些发现首次表明，rs6214“A”等位基因和“AA+AG”基因型对 NAFLD 的易感性具有保护作用。然而，需要进一步的研究来验证我们的发现。
