Department of Obstetrics, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
Central Laboratory, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
J Matern Fetal Neonatal Med. 2022 Jul;35(13):2555-2563. doi: 10.1080/14767058.2020.1789965. Epub 2020 Jul 13.
Preeclampsia (PE) and fetal growth restriction (FGR) have abnormal placental implantation and endothelial dysfunction in common. However, their etiologies are not well understood. Both heat shock protein 70 (Hsp70) and nitric oxide (NO) are suggested to play a major role in the regulation of maternal and fetoplacental hemodynamics. In this study, the association of PE with FGR and Hsp70 or NO was analyzed.
A total of 30 cases of PE, 25 cases of PE complicated with FGR and 50 cases of normal pregnant women were chose, and PE and normal animal models were constructed. Subsequently, the levels of Hsp70 and NO in serum and placental tissues of humans and animals were measured and compared. Further, rats were injected with pLV-NC-shRNA, pLV-Hsp70-shRNA, pLV-EFIa-NC, and pLV-EFIa-Hsp70, respectively, the weight of each conceptus, number of pups, fetal crown to tail length, total weight of the placenta/fetus unit, and the content of NO were analyzed.
The expression of Hsp70 in serum and placental tissues of PE complicated with or without FGR group was increased, whereas the content of NO was decreased compared to the normal group. The fetal weight (FW) of the Hsp70 targeted suppression group was higher than the other two groups, whereas the placental weight (PW) was reversed. Also, NO synthase (NOS) expression was decreased in the Hsp70 over-expression group.
We speculated that the enhancement of Hsp70 might be related to the development of PE combined with FGR through inhibiting the synthesis of NOS.
子痫前期（PE）和胎儿生长受限（FGR）具有共同的胎盘植入异常和血管内皮功能障碍。然而，它们的病因尚不清楚。热休克蛋白 70（Hsp70）和一氧化氮（NO）都被认为在调节母体和胎盘中发挥主要作用。在这项研究中，分析了 Hsp70 与 PE 和 FGR 的关系。
选择 30 例 PE、25 例 PE 合并 FGR 和 50 例正常孕妇，建立 PE 和正常动物模型。然后测量并比较了人和动物血清和胎盘组织中 Hsp70 和 NO 的水平。进一步，分别向大鼠注射 pLV-NC-shRNA、pLV-Hsp70-shRNA、pLV-EFIa-NC 和 pLV-EFIa-Hsp70，分析每个胚胎的体重、幼仔数量、胎儿冠尾长度、胎盘/胎儿单位总重量和 NO 含量。
PE 合并或不合并 FGR 组血清和胎盘组织中 Hsp70 的表达增加，而 NO 的含量降低。与其他两组相比，Hsp70 靶向抑制组的胎儿体重（FW）较高，而胎盘重量（PW）相反。此外，Hsp70 过表达组中 NOS 表达降低。
我们推测，通过抑制 NOS 的合成，Hsp70 的增强可能与合并 FGR 的 PE 的发展有关。
