B淋巴细胞慢性淋巴细胞白血病（B-CLL）细胞中的CD1表达。

CD1 expression in B-CLL cells.

作者信息

Merle-Béral H, Boumsell L, Michel A, Blanc C, Debré P

机构信息

Département d'Hématologie, Hôpital Pitié-Salpêtrière, Paris, France.

出版信息

Nouv Rev Fr Hematol (1978). 1988;30(5-6):327-30.

PMID:3265504

Abstract

The peripheral blood from 38 B-CLL patients was studied by flow cytometry with 25 clustered or not clustered monoclonal antibodies (moAbs) in order to characterize the cell surface phenotype of lymphoid cells. All moAbs were chosen since they detected B or CD1-8 T cell differentiation antigens or MHC class II antigens. The results showed a heterogeneity in the leukemic cell reactivity with the various moAbs and between patients. The restricted B cell antigens recognized by BL14, Y2955 and anti-class II moAbs were constantly expressed in leukemic cells, while B-cell antigens reacting with FMC7 and BL13 moAbs were variably detected. In addition to CD5 antigens, other T cell markers including several epitopes of the CD1 group were also found to be present on the leukemic cell surface in several cases. To further extend these data, 6 cases were selected for labelling with a battery of 6 CD1a, 2 CD1b and 1 CD1c moAbs. These results suggested that CD1a,c epitopes can be detected on leukemic B cells in addition to other T cell differentiation antigens.

摘要

运用25种聚集或未聚集的单克隆抗体（moAbs），通过流式细胞术对38例B细胞慢性淋巴细胞白血病（B-CLL）患者的外周血进行研究，以表征淋巴细胞的细胞表面表型。选择所有这些单克隆抗体是因为它们能检测B或CD1-8 T细胞分化抗原或MHC II类抗原。结果显示，白血病细胞与各种单克隆抗体的反应性以及患者之间存在异质性。BL14、Y2955和抗II类单克隆抗体识别的受限B细胞抗原在白血病细胞中持续表达，而与FMC7和BL13单克隆抗体反应的B细胞抗原则有不同程度的检出。除CD5抗原外，在一些病例中还发现白血病细胞表面存在其他T细胞标志物，包括CD1组的几个表位。为进一步拓展这些数据，选取6例患者用一组6种CD1a、2种CD1b和1种CD1c单克隆抗体进行标记。这些结果表明，除其他T细胞分化抗原外，在白血病B细胞上还可检测到CD1a、c表位。