Vuillier F, Tortevoye P, Binet J L, Dighiero G
Unité d'Immuno-Hématologie et d'Immuno-Pathologie, Institut Pasteur, Paris, France.
Nouv Rev Fr Hematol (1978). 1988;30(5-6):331-4.
Although B-CLL is a hematological disorder characterized by clonal proliferation of B-cells, in most cases an increase in absolute numbers of T cells is observed. This increase is probably polyclonal in nature, since most data indicate that T lymphocytes are not progeny of the malignant clone in B-CLL. As CD4, CD8 and NK cells correspond to heterogeneous populations, in this work we tried to better define these subpopulations. Thus, CD4 subpopulations were displayed by using double labelling techniques into helper inducer subset (CD4+CDW29+) and suppressor inducer subset (CD4+CD45R+). CD8 subpopulations were delineated according to density expression of CD8 and reactivity with CD16 and CD11b in: suppressive cells defined by high density (HD) CD8 and reactivity with CD11b; NK cells defined by low density (LD) CD8 and binding to CD16; CTL cells by LD or HD CD8 and absence of expression of CD16 and CD11b. Our results concerning 47 normal control donors and 27 B-CLLs indicated that: although percentages of cells expressing CD3, CD4, CD8 and NK markers are decreased, absolute values of these subpopulations were increased; there exists an imbalance concerning CD4 and CD8, given by a more important increase of CD8 than CD4 subpopulations, but both are significantly augmented when compared to the normal range; within CD4 subpopulations, there is an important increase of CD4 CDW29 subset (helper inducer) whereas the CD4 CD45R (suppressor inducer) is not augmented in stage A patients and decreases in stage C patients; all the 3 identified CD8 subpopulations (NK, suppressors and CTLs) appeared to be increased; in 3 cases of clonal remission CLL studied, all T cell and NK subpopulations were recovering to normal percentages.
虽然B细胞慢性淋巴细胞白血病（B-CLL）是一种以B细胞克隆性增殖为特征的血液系统疾病，但在大多数情况下，会观察到T细胞绝对数量增加。这种增加可能本质上是多克隆的，因为大多数数据表明T淋巴细胞不是B-CLL中恶性克隆的后代。由于CD4、CD8和NK细胞对应于异质群体，在本研究中我们试图更好地定义这些亚群。因此，通过双重标记技术将CD4亚群分为辅助诱导亚群（CD4+CDW29+）和抑制诱导亚群（CD4+CD45R+）。根据CD8的密度表达以及与CD16和CD11b的反应性来划分CD8亚群：高密度（HD）CD8且与CD11b反应的抑制性细胞；低密度（LD）CD8且与CD16结合的NK细胞；低密度或高密度CD8且不表达CD16和CD11b的细胞毒性T淋巴细胞（CTL）。我们对47名正常对照供体和27例B-CLL患者的研究结果表明：虽然表达CD3、CD4、CD8和NK标志物的细胞百分比降低，但这些亚群的绝对值增加；CD4和CD8之间存在失衡，表现为CD8亚群的增加比CD4亚群更显著，但与正常范围相比两者均显著增加；在CD4亚群中，CD4 CDW29亚群（辅助诱导亚群）显著增加，而CD4 CD45R（抑制诱导亚群）在A期患者中未增加，在C期患者中减少；所有3个已鉴定的CD8亚群（NK、抑制性细胞和CTL）似乎都增加了；在研究的3例克隆缓解的CLL病例中，所有T细胞和NK亚群的百分比都恢复到了正常水平。
